银杏叶提取物对脑缺血再灌注大鼠海马一氧化氮产生的影响  

Effects of Ginkgo Biloba Extract on NO Concentration in Rat Hippocampus with Transient Forebrain Ischemia and Reperfusion

作　　者：蒋敏海[1] 卢国跃[1] 顾小林[1] 华洁[1] 秦顺一 

机构地区：[1]杭州市第一人民医院神经内科,浙江杭州310006 [2]日本兵库医科大学医学物理化学教研室,日本663-8501

出　　处：《中华中医药学刊》2009年第1期55-57,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家人事部留学回国人员科技活动择优资助基金项目(2003433);杭州市科技发展计划医学重点专科专病项目(2005633Q21)

摘　　要：目的:观察银杏叶提取物(Ginkgo biloba extract)对脑缺血再灌注大鼠海马一氧化氮(NO)产生的影响。方法:大鼠脑缺血采用四血管阻断法,选择性NO测定电极检测NO的浓度。实验分为生理盐水组和银杏叶提取物组。结果:银杏叶提取物没有影响大鼠的血压和海马的血流量,显著地减少了缺血再灌流时海马内NO的产生(P<0.001)。结论:银杏叶提取物可能通过抑制一氧化氮的产生(NO)而起到神经保护作用。Objective ： To investigated the effects of Ginkgo biloba extract on nitric oxide （NO） concentration in rat hippocampus after transient forebrain ischemia. Methods ： The model of forebrain ischemia was made by 4 - vessel occlusion for 10min. NO concentration was measured directly by an NO - selective electrode method. Saline and Ginkgo biloba extract was administered intraperitoneally 20 min before the onset of ischemia. Results ： Ginkgo biloba extract did not affect the hippocampal blood flow and mean arterial blood pressure （ MABP）, compared with the saline group, Ginkgo biloba extract significantly reduced the NO production during post -ischemic early reperfusion （ P 〈 0.001 ）. Conclusion： Ginkgo biloba extract has protective effect on hippocampal neurons against cerebral ischemia - reperfusion injury may be by reducing the content of NO in hippocampus of rat.

关 键 词：银杏叶提取物 脑缺血 一氧化氮 

分 类 号：R743[医药卫生—神经病学与精神病学]