大鼠急性脑缺血后血脑屏障通透性变化的实验研究  

Experimental Study of Alteration of Blood-brain Barrier Leakage After Actite Cerebral Ischemia in Rats

作　　者：杨赞章[1,2] 陈虹[1,2] 闫明俞[1,2] 张红[3] 魏丽丽[1] 曹燕[1] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]省部共建新疆特种植物药资源教育部重点实验室,新疆石河子832002 [3]石河子大学医学院附属医院,新疆石河子832002

出　　处：《中华中医药学刊》2009年第3期501-503,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30560171)

摘　　要：目的:探讨大鼠脑缺血后血脑屏障(blood-brain barrier,BBB)通透性的变化。方法:制做永久性大脑中动脉阻塞(permanent middle cerebral artery occlusion,pMCAO)模型,之后在缺血后的不同时间点,从大鼠股静脉注射2%的伊文思蓝(Evens Blue,EB),通过荧光显微镜及甲酰胺提取两种方法来评估BBB对EB的通透性。结果:随着缺血时间的增加,脑组织EB蓝染的范围逐渐增大,荧光光斑数量增加,强度增强。甲酰胺法显示,EB的渗出量在3h时即有增加,12h达到高峰以后逐渐降低,其测定结果与荧光显微镜下的观察结果较为一致。结论:脑缺血后BBB的通透性增高,并在48h内呈单相变化。另外还发现,EB法不宜用来比较缺血区与非缺血区间BBB通透性的差异。Objective ： To study the alteration of blood brain barrier（BBB） leakage after cerebral ischemia. Methods ： The permanent middle cerebral artery occlusion model was induced in Wistar rat Subsequently,2% solution of Evans blue was administered intravenously from vena femoralis in a dose of 4mL/kg at expected time after MCAO. Then the BBB leakage was evaluated with fluorescence microscope and the method of formamide. Results ： With the prolonging of the ischemic duration, the scope of EB blue-staining in eerebral tissue increased gradually, and the number and area of fluoreseent faculae increased till 12h. The formamide-method resultimplied that the extravasation of EB inereased at 3h after MCAO,and it achieved the peak at 12h. Then it stepped down gradually till 48h after ischemia. Conclusion ：The alteration of BBB leakage during the 48h after cerebral ischemia is in a monophase. And it may be not a Correct method to evaluate the distinction of BBB leakage between ischemic region and non - ischemic region by EB.

关 键 词：血脑屏障 脑缺血 通透性 荧光显微镜 伊文思蓝 

分 类 号：R-33[医药卫生]