非嗜碱突变株的筛选  

The Selection of Unalkaliphilic Bacillus

作　　者：宋洁[1] 曹军卫[1] 涂知明 任波 钟山[1] 

机构地区：[1]武汉大学生命科学院微生物及免疫学教研室

出　　处：《湖北医科大学学报》1998年第2期106-108,共3页

摘　　要：以带有Ｔｎ５的大肠杆菌（Ｔｎ５ＧｍｌＱβλ：Ｆ’ｌａｃ＋ｐｒｏ＋／△ｌａｃｐｒｏｖａｌｒ）作为供体。兼性嗜碱细菌ＮＴＴ８９（ＫｍｓＡｍｐｒ）为受体，采用滤膜杂交的方法，然后在含有卡那霉素和氨苄青霉素的平板上得到双抗菌落，可得出Ｋｍｒ的转移频率远远大于供受体菌的自发突变率，然后把得到的双抗菌落进行筛选，获得５株非嗜碱性突变株。? E Coli 142 with Tn5 as donor, facultative alkaliphillic bacillus NTT89 as receptor and with filter hybridization, the colonies which are resistant to Km and Amp on the plates were obtained in the experiment. From these colonies, the unalkaliphilic mutation Bacillus which do not produce on alkaline plates on resistant plates were selected.

关 键 词：芽孢杆菌属 遗传学 诱变性试验 嗜碱性 

分 类 号：R378.7[医药卫生—病原生物学]