成年态柑橘离体植株中特异表达蛋白的初步研究  

Primary Study on the Specifically Expression Proteins in In vitro Cultures of Adult Citrus

作　　者：王彩霞[1,2] 罗丽[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东青岛266109 [2]华中农业大学植物科学技术学院,武汉430070

出　　处：《中国农学通报》2009年第7期70-72,共3页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"三峡库区柑桔非疫区建设核心技术开发研究"(2007BAD47B03-2);青岛农业大学"高层次人才启动基金"(630812)。

摘　　要：利用SDS-PAGE技术分析了不同培养时期成年态柑橘鱼橙离体植株中蛋白质的变化动态。结果表明,组织培养初期,总蛋白表达量略有降低,之后逐渐升高并趋于稳定。分子量约为21kDa和31kDa的蛋白在继代培养至第2次时,表达量开始增加,其中前者仅在柑橘离体植株中特异表达。通过改进的透析袋电洗脱法对分子量约21kDa的蛋白回收纯化后利用质谱进行初步鉴定。结果显示,该特异表达蛋白与数据库中报道的蛋白同源性均低于50%,因此推测该蛋白属功能未知的新蛋白,其在柑橘组织培养过程中的作用有待于进一步研究。Dynamic changes of proteins in in vitro cultures of Fisher navel over periodical sub-culturings were analyzed with SDS-Polyacrylamide gel electrophoresis. Results showed that the quantity of total expression proteins in the early in vitro cultures were lower than that in field plant, however, it increased and trended to stability in the later in vitro cultures of Fisher navel. The expression quantity of 21 kDa and 31 kDa proteins were increased when sub-cultured for the second cycles, and the former protein was expressed specifically in citrus. The protein of 21 kDa was also identified by mass spectrum after it was purified by modified electric elution method aided with dialysis tube. Results indicated that the sequence similarity of this protein compared with all that of proteins published in database was lower than 50%. It was suggested that this protein was a novel protein with no information about its functions, so that much work remains for realizing the effect of the protein on in-vitro culture of Fisher navel in the process of sub-culturings.

关 键 词：柑橘 离体植株 SDS-PAGE 特异表达 蛋白纯化 

分 类 号：S666[农业科学—果树学]