自身免疫性肝炎患者血清中高迁移率族蛋白B1检测及临床意义  

Detection high mobility group box-1 protein in autoimmune hepatitis

作　　者：赵擎[1] 辛绍杰[2] 侯俊 胡燕 貌盼勇[3] 

机构地区：[1]中国人民解放军总医院南楼消化科,北京100853 [2]解放军第三0二医院传染三科,北京100039 [3]解放军第三O二医院传染病研究所病毒研究室,北京100039

出　　处：《临床肝胆病杂志》2009年第2期118-119,共2页Journal of Clinical Hepatology

摘　　要：目的采用ELISA检测方法,初步探讨人高迁移率族蛋白B1(HMGB1)与自身免疫性肝炎(AIH)发病的相互作用关系。方法将纯化的HMGB1蛋白,包被ELISA检测微孔板,检测AIH患者和健康献血员的血清分别165份和69份,分析比较AIH患者和健康献血员的血清中抗HMGB1抗体的分泌情况。结果AIH患者血清中抗HMGB1抗体的阳性率明显高于健康献血员(78.7%VS50.7%,P<0.05)。直线回归分析显示,抗HMGB1抗体滴度(OD值)与ALT水平有很好的相关性(相关系数r=0.968)。结论HMGB1是AIH疾病发生发展中一个重要的炎症蛋白,与疾病的炎症活动程度有很好的相关性。Objective Adaption the method of ELISA, investigation the relationship between the human high mobility group box-1 protein （HMGB1） and autoimmune hepatitis （AIH）. Methods Coating the microwell plate using purified HMGB1 protein, detection the serunl of the 165 AIH and 69 healthy respectively. Comparison the level of Anti - HMGB1 antibody in AIH and the healthy. Results The positive ratio of Anti - HMGBI antibody in AIH more than that in healthy （78.7% VS 50. 7% ,P 〈 0. 05 ）, The linear regression analysis showed there were good correlation between the anti - HMGBI antibody titer and the level of alanine transarninase （ALT） （correlation coefficient, r = 0. 968 ）. Conclusion HMGB1 is a crucial inflammaton./protein in the progressing of AIH, there are good correlation between the anti - HMGB1 antibody titer and the stage of inflammation in AIH.

关 键 词：高迁移率族蛋白B1 自身免疫性肝炎 ELISA 

分 类 号：R575.1[医药卫生—消化系统] R446.6[医药卫生—内科学]