plk1、Raf-1和Ki-67在胃癌组织中的表达及其意义  

Expression of plk1,Raf-1 and Ki-67 in Human Gastric Neoplasm and its Significance

作　　者：陈壮威[1] 戴起宝[2] 兰斌[2] 张洪生[3] 陈绍勤[2] 高爱明[3] 

机构地区：[1]福建省人民医院肿瘤科,福州350004 [2]福建医科大学附属第一医院肿瘤科,福州350005 [3]福建省人民医院病理科,福州350004

出　　处：《福建医科大学学报》2009年第2期121-124,共4页Journal of Fujian Medical University

基　　金：福建省卫生厅青年基金(2005-2-58)

摘　　要：目的探讨plk1、Raf-1和Ki-67在胃癌组织中的表达及其相关性,分析它们的表达与胃癌临床病理指标之间的关系。方法采用免疫组织化学SP法检测80例胃癌组织和15例癌旁正常胃组织中plk1、Raf-1和Ki-67的表达水平。结果80例胃癌组织中plk1、Raf-1和Ki-67的阳性表达率分别为82.5%(66/80)、81.3%(65/80)、80.0%(64/80),15例癌旁正常胃组织中均未见表达。plk1和Raf-1表达与胃癌的浸润深度、TNM分期以及淋巴结转移显著相关(P<0.05),与患者性别、年龄、肿瘤部位、分化程度无相关性;Ki-67表达与胃癌的分化程度、浸润深度、TNM分期以及淋巴结转移显著相关(P<0.01),而与其它因素无关。plk1与Ki-67、Raf-1与Ki-67、plk1与Raf-1的表达显著相关(P<0.01)。结论plk1、Raf-1和Ki-67的过表达可能与胃癌的发生发展有关,三者联合检测可为判断胃癌生物学行为乃至预后提供重要的参考指标。Objective To investigate the expressions of ptkl,Raf-land Ki-67 in gastric neoplasms and their relationships, and to analyse the relationship between their expressions and clincoipathological parameters. Methods Immunohistochemical techniques were employed to detect the expressions of plkl, Raf-1 and Ki-67 in 80 cases of human gastric neoplasms and 15 cases of adjacent normal gastric tissues. Results （1）The positive rates of plkl, Raf-1 and Ki-67 expressions in gastric neoplasms tissues were 82.5%（66/80）, 81.3%（65/80） and 80.0%（64/80） respectively while the plkl, Raf-1 and Ki-67 expressions in 15 adjacent normal gastric tissues all showed negative. （2）The expressions of plkl and Raf-1 in gastric neoplasms were significantly relevant to the depth of tumor invasion, TNM stage, and lymph node metastasis（P〈0.05）but not with other parameters; The expression of Ki-67 in gastric neoplasms was significantly correlated with histological differentiation, depth of tumor invasion, TNM stage, and lymph node metastasis（P〈0. 01）but not with other parameters. （3）The expressions of plkl and Raf-1 were significantly correlated, so were those of Raf-1 and Ki-67, and those of plkl and Ki-67（P〈0.01）. Conclusion The overexpressions of plkl, Raf-1 and Ki-67 are related to tumorgenesis and the development of gastric neoplasms, and could be regarded as important indicators in the identification of biological characteristics and prognosis of gastric neoplasms.

关 键 词：胃肿瘤 KI-67抗原 蛋白激酶类 免疫组织化学 

分 类 号：R735.202.2[医药卫生—肿瘤] R977.6[医药卫生—临床医学]