一条家族性急性髓系白血病相关新基因ELF2C的生物信息学分析  

Bioinformatics Analysis of ELF2C,a Novel Gene Related to Damilial Acute Myelogenous Leukemia

作　　者：王程毅[1] 王少元[1] 张轶文[1] 李景岗[1] 

机构地区：[1]福建医科大学附属协和医院血液科,福建省血液病研究所

出　　处：《福建医科大学学报》2009年第2期137-141,共5页Journal of Fujian Medical University

基　　金：国家自然科学基金(30770909);福建省重大科研项目(2003F003)

摘　　要：目的利用生物信息学技术对新克隆的家族性急性髓系白血病相关新基因ELF2C和其蛋白序列进行分析,初步探讨其功能。方法以人类基因组数据库为基础,利用生物信息学程序预测ELF2C的基因结构、染色体定位、编码蛋白质的理化性质、亚细胞定位、蛋白质功能域等。结果ELF2C基因定位于4q31.1,全长2257bp,含有432bp的开放阅读框,可编码143氨基酸的蛋白质,且编码蛋白定位于核内,具有多个修饰位点和功能基序,是一个功能活跃的蛋白质。结论ELF2C基因可能是在家族性急性髓系白血病发生发展中具有生物功能的一条全长新基因。Objective To analyze the sequence of a novel familial acute myelogenous leukemia （AML） related gene ELF2C and its encoding protein product, and to explore its potential functions in familial AML. Methods Based on the human genome resources,the gene structure and chromosomal location of ELF2C, its encoding protein＇s biochemical features, subeellular localization and functional domains were predicted by applying bioinformatics analysis tools （MegaBLAST, BLAT Search Genome, SIM4, GeneBuilder, ProtParam, PSORT Ⅱ, TMHMM, InterProScan, SMART, ELM）. Results ELF2C is located on the human chromosome 4q31.1, with a total length of 2 257 bp containing a 432 bp open reading frame （ORF） encoding a protein with 143 amino acids. The ELF2C protein is localized to the nucleus. It contains several structural domains and post-translational modification sites, which may be related to its specific biological functions. Conclusion From the results of bioinformatics analysis, the novel gene ELF2C may play an important role in the onset and development of familial AML.

关 键 词：系谱 白血病 粒细胞.急性 基因 计算生物学 序列分析 DNA 

分 类 号：Q987[生物学—遗传学]