靛玉红甲肟对人食管癌细胞株EC-1和Kyse70体外增殖和细胞周期的影响  

Effects of indirubin-3'-monoxime on proliferation and cell cycle of human EC-1 and Kyse70 esophageal cancer cell

作　　者：陈小兵[1,2] 罗素霞[1,2] 张军辉[1] 刘晓莉[2] 黄幼田[3] 索振河[1] 

机构地区：[1]郑州大学第一附属医院肿瘤内科,郑州450052 [2]河南省肿瘤医院肿瘤内科,郑州450008 [3]郑州大学基础医学院病理生理教研室,郑州450052

出　　处：《肿瘤》2009年第4期350-353,共4页Tumor

基　　金：卫生部科学研究基金-河南省医学科技攻关计划项目(编号:WKJ2007-2-026)

摘　　要：目的:探讨靛玉红甲肟(indirubin-3'-monoxime)对体外培养的人食管癌细胞株EC-1和Kyse70细胞增殖和细胞周期的影响。方法:不同浓度靛玉红甲肟处理食管癌EC-1和Kyse70细胞后,采用MTT法检测细胞的增殖活性,FCM法和RT-PCR法检测细胞周期分布及bcl-2和bax mRNA表达的变化。结果:靛玉红甲肟对人食管癌细胞EC-1和Kyse70的生长具有明显的抑制作用,且表现为剂量依赖性和时间依赖性(P<0.05)。FCM法检测发现,以5μmol/L的靛玉红甲肟对EC-1细胞处理24、48和72h后,G0/G1期细胞比例逐渐下降(P<0.05),S期细胞比例未见明显改变,G2/M期细胞比例逐渐上升(P<0.05),呈时间依赖性。RT-PCR法检测发现人食管癌细胞bcl-2和bax mRNA比例呈时间依赖性下调。结论:靛玉红甲肟对人食管癌细胞EC-1和Kyse70具有明显的增殖抑制作用。Objective： To explore the effects of indirubin-3＇ -monoxime on proliferation and cell cycle of human esophageal cancer cell line EC-1 and Kyse70 cells. Methods： EC-1 and Kyse70 cells were treated with different concentrations of indirubin-3＇ -monoxime. The proliferative status of esophageal cancer cells was measured by MTT assay. Cell cycle distribution and expression of bcl-2 and bax mRNA were analyzed by flow cytometry （FCM） and RT-PCR, respectively. Results： Indirubin-3 ＇ -monoxime markedly inhibited the growth of human esophageal cancer EC-1 and Kvse70 cells in a dose- and time-dependent manner （ P 〈 0.05 ）. FCM analysis found that the proportion of cells in G0/G1 phase decreased gradually at 24, 48, and 72 h after treatment with 5 μmol/L indirubin-3 ＇ -monoxime （P 〈0.05）, the ratio in S phase had no change, cells in G2/M phase increased gradually （P 〈0.05）. The effects were in a timedependent manner. RT-PCR showed that the ratio of bcl-2 to bax mRNA was downregulated in a time-dependent manner. Conclusion： Indirubin-3 ＇ -monoxime markedly inhibited the proliferation of hmnan esophageal cancer lines EC-1 and Kyse70. The action mechanism may be related with G2/M phase arrest and down-regulation of the ratio of bcl-2 to bax.

关 键 词：食管肿瘤 细胞周期 细胞增殖 靛玉红甲肟 基因 BCL-2 

分 类 号：R735.1[医药卫生—肿瘤]