盐胁迫下两个亚麻品种幼苗的生理生化特性  

Physiological and Biochemical Characteristics to Salt Stress on Shoots of Two Flax Cultivars

作　　者：李文婷[1] 姜丽[1] 计巧灵[1] 苗书魁[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《生物技术》2009年第2期26-29,共4页Biotechnology

基　　金：国家自然科学基金项目资助("亚麻与罗布麻体细胞杂交及其种质的耐盐性遗传研究";30660095);新疆教育厅重点科研项目资助("亚麻与罗布麻体细胞杂交育种研究";XJEDU2006108)

摘　　要：目的:探讨两个亚麻品种(‘双亚5号’和‘范妮’)幼苗对盐胁迫的生理反应。方法:当‘双亚5号’和‘范妮’的种苗长出第二片真叶时,对其进行不同浓度的NaCl胁迫(0、70、100、150、200、250mmol/L),处理7d后研究其不同盐浓度胁迫下的丙二醛含量、过氧化物酶和过氧化氢酶活性、酯酶同功酶和过氧化物酶同功酶谱带变化。结果:获得了盐胁迫下‘双亚5号’和‘范妮’幼苗的生理生化数据和图谱。结论:在100mmol/LNaCl胁迫后,‘双亚5号’幼苗POD和CAT酶活性均达到峰值;在200mmol/L和100mmol/LNaCl胁迫后,‘范妮’幼苗的POD和CAT酶活性分别达到最大值。对于各组处理,‘范妮’幼苗的POD和CAT酶活性均明显高于‘双亚5号’幼苗的。因此,认为‘范尼’幼苗比‘双亚5号’幼苗更具耐盐潜力。Objective：It was to search the physiological and Biochemical Characteristics to salt stress on shoots of two flax cultivars （‘shuangya No.5＇ and ‘fany＇）. Method： When the second euphylis of ‘shuangya No.5＇ and ‘fany＇ came out, responses of shoots to NaCl stress were studied in control （0mmol/L） and salt-stressed （50,70,100,150 and 200mmol/L） for 7 days in terms of MDA content, peroxidase （POD） and catalase （CAT） activities, peroxidase （POD） isozyme and esterase isozyme electrophoretic bands. Result： The relative physiological and biochemical data of the two flax cultivars shoots under salt stress, as well as zymograms, were gained. Conclusion： The activities of peroxidase （POD） and catalase （CAT） of ‘shuangya No.5＇ reached peak value after treatment with 100mmol/L NaCl, As for shoots of ‘Fany＇, the POD and CAT activities reached maximum after treatment with 200mmol/L and 100mmol/L NaCl respectively. The POD and CAT activities of ‘Fany＇ shoots were all higher than those of ‘shuangya No.5＇ for NaCl stress in the corresponding levels of different concentrations. Therefore, it was concluded that the shoots of ‘Fany＇ were better than that of ‘shuangya No.5＇ for the salt resistant potential.

关 键 词：'双亚5号’幼苗 '范妮’幼苗 NACL胁迫 丙二醛含量 POD活性和POD同功酶 CAT活性 酯酶同功酶 

分 类 号：Q945[生物学—植物学]