敌敌畏降解菌的分离鉴定及降解特性研究  

Isolation and Identification of a Bacterium DDW-1 Capable of Degrading DDVP and Its Degrading Characteristics

作　　者：蔡颖慧[1,2] 张惠文[1] 苏振成[1] 张晓黎[1,2] 李新宇[1] 

机构地区：[1]中科院沈阳应用生态研究所,辽宁沈阳110016 [2]中国科学院研究生院,北京100049

出　　处：《生物技术》2009年第2期59-62,共4页Biotechnology

基　　金：绿色农业科学研究与示范项目(2007-3);国家青年科学基金项目(40701088)资助

摘　　要：目的:从受有机磷农药污染的土壤中分离能降解DDVP的菌株,对其进行鉴定和降解特性研究。方法:采用DDVP为惟一碳源和能源的无机盐培养基,通过富集培养、平板划线分离得到一株优势菌,编号为DDW-1,采用形态学、生理生化和16S-rDNA序列分析对其进行鉴定,采用气相色谱测定菌株DDW-1对DDVP的降解能力,并进行底物广谱性测试和降解酶定位实验。结果:该菌株鉴定为甲基杆菌属(Methylobacterium sp.)。降解特性试验结果表明,其最佳生长条件为温度28℃,初始pH为7.0,在该条件下,500mg·L-1DDVP经过DDW-1菌株代谢3d后,降解率达63.7%。结论:菌株DDW-1能降解DDVP,该菌株产胞内酶。Objective：A DDVP-degrading bacterium was isolated from the long-term organophosphorous pesticide-polluted soil. Then the bacterium was identified and its degrading capability was studied. Method： Utilizing DDVP as the sole carbon and energy source, a predominated bacterium,designated as strain DDW-1,was isolated by enrichment culture and streak plate method.The strain DDW-1 was identified based on 16S rDNA sequence analysis and its morphological and physiological characteristics and its DDVP degradation capability was measured by gas chromatography. Result： The strain DDW-1 was identified as Methylobacterium sp. The optimum conditions for the growth of the strain DDW-1 were initial pH 7.0 and 28℃. After 3 days, DDVP was degraded by 63.7%under the optimum condition. Conclusion： DDVP could be degraded by strain DDW-1 and some endoenzyme was produced during this process.

关 键 词：DDVP 降解菌 筛选 鉴定 DDW-1 

分 类 号：X172[环境科学与工程—环境科学]