响应面试验设计优化脱氢酶发酵培养基  

Application of Response Surface Methodology in Optimizing the Fermention Conditions for Producing Dehydrogenase

作　　者：李静文[1] 路福平[1] 别松涛[1] 张业尼[1] 张艺[1] 刘瑞娟[1] 

机构地区：[1]天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《生物技术》2009年第2期73-76,共4页Biotechnology

基　　金：国家科技基础条件微生物菌种资源平台项目(2005DKA21204-10);天津科技大学引进人才科研启动基金项目(20080417)资助

摘　　要：目的:对简单节杆菌TCCC11037发酵生产脱氢酶的培养基进行优化。方法:利用单因子试验筛选出最适碳源为葡萄糖,氮源为酵母膏。采用Plackett-Burman(P-B)方法筛选出对产酶有重要影响的因素,并采用响应面试验设计(RSM)对重要因素进行优化。结果:葡萄糖、酵母膏、KH2PO4的浓度是影响脱氢酶产生的重要因素。优化后的培养基组成为(%):葡萄糖1.21,酵母膏0.65,KH2PO40.24,玉米浆0.8;培养基初始pH值7.0,接种量5%,通气条件为装液量100mL/500mL。结论:优化后脱氢酶活力达到24.57μmol/(g·min),得到了明显的提高。Objective：Optimize the fermention conditions of dehydrogenase by Arthrobacter simplex TCCC11037. Method： By single-factor test, the optimum carbon source and nitrogen source were confirmed as glucose and yeast extract respectively. The important factors were selected by Plackett-Burman method, and response surface analysis was used to optimize these important factors. Result： The dehydrogenase activity was mainly influenced by concentration of glucose, yeast extract and KH2PO4. The optimal fermention medium components（%） were： glucose 1.21, yeast extract 0.65, KH2PO4 0.24, corn steep liquor 0.08, the optimum enzyme producing conditions were： initial pH of medium 7.0, inoculum volume 5%, liquid volume in flask 100mL/500mL. Conclusion： At optimum conditions, the dehydrogenase activity was significantly improved to 24.57μmol/（g·min）.

关 键 词：简单节杆菌 脱氢酶 PLACKETT-BURMAN 优化 响应面 

分 类 号：TQ464.8[化学工程—制药化工]