胆道良、恶性病变p53和bcl-2的表达及意义  

Gene expression significance of p53, bcl 2 in human biliary tract benign and malignant disease

作　　者：张卫[1,2,3] 钱光相[1,2,3] 孟荣贵[1,2,3] 张秀忠 

机构地区：[1]第二军医大学长海医院普通外科 [2]第二军医大学东方肝胆外科医院胆道外科 [3]第二军医大学东方肝胆外科医院病理科

出　　处：《第二军医大学学报》1998年第2期140-142,共3页Academic Journal of Second Military Medical University

摘　　要：目的：研究人胆道的良、恶性病变中凋亡抑制基因ｐ５３和ｂｃｌ－２的表达及其意义。方法：３３例胆囊腺癌及１５例胆管癌石蜡包埋组织切片，经免疫胶体金方法（ＩＧＳＳ）染色，并在显微镜下计数阳性细胞。结果：８４．８％（２８／３３）的胆囊腺癌，６６．７％（１０／１５）的胆管癌ｐ５３蛋白阳性，两者间有显著性差异（Ｐ＜０．０５）。６０．６％（２０／３３）的胆囊腺癌，６０．０％（９／１５）的胆管癌ｂｃｌ－２阳性，两者间无差异。胆囊炎、纤维间隔及正常组织ｐ５３及ｂｃｌ－２表达均为阴性。ｂｃｌ－２阳性组中代表细胞增殖活性的ｋｉ－６７指数显著高于ｂｃｌ－２阴性组（胆囊腺癌Ｐ＜０．０５，胆管癌Ｐ＜０．０１）。ｐ５３的表达与ｋｉ－６７指数无相关性。结论：ｂｃｌ－２和突变型ｐ５３对肿瘤细胞凋亡的抑制在胆道腺癌的发生、发展中共同发挥作用，但ｂｃｌ－２和ｐ５３表达与肿瘤的预后无明显关系。Objective: To study the expression of apoptosis suppressor gene p53 and bcl2 in benign and malignant disease of human biliary duct and its significance. Methods: Paraffin sections in 33 cases of gallbladder carcinoma and 15 cases of biliary duct carcinoma were examined using immunogold silver staining(IGSS),and then positive cells were detected under light microscope. Results:The positive rates of p53 were 84.8%(28/33) in gallbladder carcinoma and 66.7% (10/15) in biliary duct carcinoma, and there was statistically significant difference(P<0.05).The positive rates of bcl2 were 60.6%(20/33) in gallbladder carcinoma and 60.0%(9/15) in biliary duct carcinoma, and there was no statistically significant difference. p53 and bcl2 were negative in all adjacent normal tissue, stromal fibroblasts and cholecystitis . bcl2 expression was associated with increased cell proliferation activity(P<0.01). Conclusion: p53 and bcl 2 play a role in the occurrence and development of biliary tract carcinoma,but there is no relationship between p53 or bcl2 expression and prognosis.The higher the ki67 index, the shorter the servival time.

关 键 词：胆道病变 良性 恶性 P53基因 BCL-2基因 

分 类 号：R735.802[医药卫生—肿瘤]