分子克隆技术制备miniSTR基因座等位基因分型标准物  

Construction of Standard Allelic Ladder of miniSTR Loci by Molecular Cloning

作　　者：白雪[1] 丛斌[1] 李淑瑾[1] 侯志平[1] 谷建立[1] 刘宁[1] 李霞[1] 郭霞[1] 

机构地区：[1]河北医科大学法医学系河北省法医学重点实验室,河北石家庄050017

出　　处：《法医学杂志》2009年第2期106-108,共3页Journal of Forensic Medicine

基　　金：河北省科技支撑计划项目(072461479D)

摘　　要：目的调查D9S2157、D9S1122、D10S1435、D12ATA63和D2S1776共5个miniSTR基因座在河北汉族人群中的遗传多态性,并用分子克隆的方法制备等位基因分型标准物。方法用荧光PCR和ABI310遗传分析仪对河北汉族120份无关个体血样中的5个miniSTR基因座进行遗传多态性分析。用分子克隆的方法构建等位基因分型标准物。结果5个基因座在河北汉族人群中分别检测出了8、8、7、5和8个等位基因,多态信息含量分别为0.790、0.720、0.750、0.630和0.850。结论5个基因座在河北汉族人群中有较高的多态信息含量,其分子克隆法制备的等位基因分型标准物具有较高的法医学应用价值。Objective To investigate the genetic polymorphism of five miniSTR loci（D9S2157, D9S1122, D10S1435, D12ATA63, D2S1776） in Hebei Han population and to construct standard allelic ladders. Methods Polymorphism of the five miniSTR loci in 120 unrelated individuals was analyzed by fluorescence PCR and ABI 310 Genetic Analyzer. Molecular cloning technique was employed to construct standard allelic ladder of the 5 loci. Results Of the five miniSTR loci, 8, 8, 7, 5 and 8 alleles were found, respectively. The polymorphism information component were 0.790, 0.720, 0.750, 0.630 and 0.850, respectively. Conclusion The five loci have relatively abundant polymorphic information and their standard allelic ladders constructed by molecular cloning technique are useful in forensic science .

关 键 词：法医遗传学 多态现象 遗传 克隆 分子 MINISTR 等位基因分型标准物 

分 类 号：R394[医药卫生—医学遗传学] R392.11[医药卫生—基础医学]