3个miniSTR基因座在湖南汉族人群中的遗传多态性  

Polymorphism of D10S1248, D2S441, D1S1677 in Hunan Han Population

作　　者：李霞[1] 丛斌[1] 李淑瑾[1] 白雪[1] 许立[1] 侯志平[1] 郭霞[1] 

机构地区：[1]河北医科大学法医学系河北省法医学重点实验室,河北石家庄050017

出　　处：《法医学杂志》2009年第2期112-114,共3页Journal of Forensic Medicine

基　　金：河北省"十一五"科技支撑计划项目(072461479D)

摘　　要：目的建立扩增片段小于120bp,包括D10S1248、D2S441和D1S16773个miniSTR基因座的复合扩增系统,并调查其在湖南汉族人群中的遗传多态性。方法采用不同荧光染料标记引物,通过PCR扩增,利用ABI310遗传分析仪对186份无关个体血样进行3个miniSTR基因座片段长度分析。结果D10S1248、D2S441和D1S1677miniSTR基因座均获得了清晰的基因分型结果,经对186名无关个体进行分析,分别检出9、7、7个等位基因和21、19、15种基因型,基因型分布符合Hardy-Weinberg平衡。3个基因座在湖南汉族人群的非父排除率和个体识别力分别为0.465、0.491、0.361和0.886、0.899、0.818。结论建立的3个miniSTR基因座扩增系统在DNA高度降解检材分析中具有较高的应用价值,并且在湖南汉族人群中具有较好的遗传多态性,可应用于个体识别和亲权鉴定。Objective To establish a multiplex set including D10S1248, D2S441, D1S1677 and to investigate the genetic polymorphism of the three miniSTR in Hunan Hun population. Methods Three miniSTR loci of 186 unrelated individuals were amplified with different multiplex fluorescence-labeled primers. The amplified products were analyzed by ABI 310 Genetic Analyzer to identify genotype. Results Each locus was successfully genotyped. Among the 186 individuals investigated, 9, 7 and 7 alleles, as well as 21, 19 and 15 genotypes were detected at D10S1248, D2S441and D1S1677, respectively. No significant deviation from Hardy-Weinberg equilibrium was observed. The excluding probability of paternity and the discrimination power were 0.465, 0.491 and 0.361, as well as 0.886, 0.899 and 0.818 for D10S1248, D2S441 and D1S1677, respectively. Conclusion The miniSTR multiplex set can benefit forensic analysis of degraded samples. It has shown good polymorphism in Hunan Han population and can be used in personal identification and paternity test.

关 键 词：法医遗传学 多态现象 遗传 MINISTR 湖南 汉族 

分 类 号：R394[医药卫生—医学遗传学] R814.4[医药卫生—基础医学]