放射线治疗对小鼠肾癌肿瘤坏死因子-α表达的影响  

Effect of radiotherapy on expression of TNF-α in mouse renal carcinoma

作　　者：吕海燕[1] 黄建华[1] 王瑶[1] 郭佳[1] 郭业磊[1] 俞伟[2] 马林[2] 

机构地区：[1]解放军总医院肿瘤免疫治疗研究中心,北京100853 [2]解放军总医院放射治疗科,北京100853

出　　处：《军医进修学院学报》2009年第2期176-177,196,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：北京市自然科学基金资助项目(7042060)~~

摘　　要：目的:观察放射治疗对BALB/c小鼠肾癌抑制作用及对TNF-α表达的影响。方法:用Renca肾癌细胞制作BALB/c小鼠皮下移植瘤模型,放疗组小鼠在种瘤第12～16天,连续5d进行肿瘤局部放射,第28天处死小鼠,取肿瘤组织测量,分析治疗效果。体外培养2×106Renca肾癌细胞,经20GyX射线单次照射培养24h后,收获细胞。提取细胞和肿瘤组织蛋白,免疫印迹法检测治疗后肿瘤组织和细胞中TNF-α蛋白的表达水平。结果:放射治疗组有效抑制肾癌细胞在BALB/c小鼠体内生长,局部放疗明显增加了肿瘤组织内TNF-α表达水平,体外培养的Renca肾癌细胞经放射线处理后TNF-α表达明显上调。结论:放射线能够促进肿瘤细胞分泌TNF-α,并且与肿瘤局部产生的TNF-α表达上调密切相关。Objective： To observe the inhibitory effect of radiotherapy on renal carcinoma and expression of TNF-α in BALB/c mice. Methods： Renal carcinomabarring mice were treated with local fractionated tumor irradiation. The mice were euthanized on day 28. Cultured Renca cells were exposed to a single radiation dose of 20Gy after cultured for 24h. Expression of TNF-α protein in tumors and Renca cells was detected by Western blot. Results： Radiotherapy inhibited the growth of renal carcinoma in BALB/c mice. Local radiotherapy significantly increased the expression of TNF-α in tumor tissue. The expression of Renca cells cultured in vitro was significantly up-regulated after radiotherapy. Conclusion： X-rays can stimulate tumor tissue to secrete TNF-α and are correlated with the up-regulation of TNF-α expression in tumor tissue.

关 键 词：肾肿瘤 放射疗法 计算机辅助 肿瘤坏死因子-Α 

分 类 号：R737.11[医药卫生—肿瘤]