家蝇抗菌物质诱导及在不同pH下的抑菌活性  

Antibacterial activity of antibacterial substances induced in the housefly at different pH levels

作　　者：许兵红[1] 李超英[1] 陈萍[1] 李进芬[1] 

机构地区：[1]新乡医学院寄生虫学教研室,河南新乡453003

出　　处：《中国病原生物学杂志》2009年第3期209-210,223,共3页Journal of Pathogen Biology

基　　金：河南省教育厅自然科学基础研究项目(No.2007310010)。

摘　　要：目的探讨家蝇幼虫经针刺诱导产生的抗菌物质在不同pH条件下的抑菌活性。方法室内饲养至3龄的家蝇幼虫经针刺诱导48 h后提取血淋巴,平板法作抑菌试验,观察不同pH条件下对溶壁微球菌和金黄色葡萄球菌的抑菌效果。结果针刺诱导产生的抗菌物质对溶壁微球菌的抑菌环直径pH6.5时最大,为(25.17±0.29)mm,随pH继续增高后抑菌环直径减小;对金黄色葡萄球菌的抑菌环直径在pH5.5和6.0时最大,均为(12.83±0.58)mm,pH继续增高后抑菌环直径减小。结论家蝇幼虫经针刺诱导产生的抗菌物质在弱酸性条件下活性较强,随pH增高活性减弱。Objective To investigate the antibacterial activity induced in larvae of the housefly by pricking at different pH levels.Methods After larvae were pricked for 48 h,hemolymph was collected from the surviving larvae and was tested against Micrococcus lysodeikticus and Staphylococcus aureus under different pH conditions.Diameters of clear inhibitory rings were observed and recorded to express antibacterial activity.Results The largest antibacterial ring was found at pH6.5 in a group tested against M.lysodeikticus.Similarly,this ring was found at pH5.5 and 6.0 in groups tested against S.aureus.The diameter of the antibacterial ring decreased along with an increase in pH.Conclusion Findings suggest that the antibacterial activity of antibacterial substances induced by pricking in the housefly is related to pH.This activity is greatest under slightly acid conditions.

关 键 词：家蝇 抗菌物质 诱导 PH 

分 类 号：R384.2[医药卫生—医学寄生虫学] R378[医药卫生—基础医学]