X射线诱发外周血淋巴细胞TCR基因突变研究  

X-ray induced TCR gene mutation of peripheral blood lymphocytes

作　　者：侯殿俊[1] 马娅[1] 刘伟[1] 乔建维[1] 卢峰[1] 商希梅[1] 李洁清[1] 封丽[1] 

机构地区：[1]山东省医学科学院放射医学研究所,济南250062

出　　处：《中华放射医学与防护杂志》2009年第2期151-154,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：山东省自然科学基金（Y2007C151）

摘　　要：目的用培养法研究X射线诱发的人外周血淋巴细胞TCR基因突变情况。方法以不同剂量（0．8Gy）的X射线照射新鲜分离的健康成人外周血淋巴细胞，植物血凝素、白细胞介素2（IL-2）协同刺激培养7d，流式细胞术检测TCR基因突变频率（TCRMF），并拟合剂量效应关系。结果随着照射剂量的增加，TCR基因突变频率随之上升，最佳拟合曲线为二次多项式模型。结论TCR基因突变可作为辐射生物剂量计，用于急性辐射照射生物剂量的估算。Objective To study the TCR gene mutation in peripheral blood lymphocytes induced by X- ray exposure using cultured lymphocytes cloning method. Methods Freshly isolated peripheral lymphocytes from healthy adult donors were irradiated with X-ray in doses ranging from 0 to 8 Gy and cultured with interleukin2 and phytohemagglutinin for 7 days. The mutant frequencies of TCR gene （TCR MF） were detected by flow eytometry and the dose response curves were fitted. Results TCR MF increased with the dose going up. An aquadratic polynomial dose response model was fitted. Conclusions TCR gene mutation could which serve as a potential biological dosimeter. It might be applied for the estimation of biological close in emergency exposure.

关 键 词：辐射照射 基因突变 TCR基因 生物剂量计 

分 类 号：R144[医药卫生—公共卫生与预防医学]