ATP酶在细菌潜生体相关的IBS大鼠模型肠黏膜中的变化  

Changes of intestinal mucosa ATPase in the bacterial cryptic growth cell-related IBS rat model

作　　者：裴轶劲[1] 黄梅[1] 郭林明[1] 吴小兰[1] 刘俊康[1] 

机构地区：[1]中国人民解放军第三军医大学医学检验系与药学院生物波研究室,重庆市400038

出　　处：《世界华人消化杂志》2009年第8期817-820,共4页World Chinese Journal of Digestology

基　　金：重庆市科委自然科学基金计划基金资助项目;No.CSTC2006BB5068~~

摘　　要：目的:研究细菌潜生体相关的肠易激综合征(irritable bowel syndrome,IBS)大鼠模型肠黏膜ATP酶的变化.结论:IBS大鼠回盲部黏膜ATP酶活性显著降低,与能量代谢降低相关,这可能是引起肠黏膜屏障功能受损的重要原因,与IBS病因相关.AIM： To study the ATPase changes of intestinal mucosa of the bacterial cryptic growth cell （CGC）-related irritable bowel syndrome （IBS） rat model. METHODS： Wistar rats were divided into nor- mal control group and the bacterial CGC-related IBS rat model group. The ATPase activity in rat ileocecal mucosa was tested by inorganic phos- phorus detection method, the energy charge （Ec） of adenosine triphosphate and the ratio of ATP to the total amount of adenylic acid pool in ileo- cecal mucosa cells were detected by high perfor- mance liquid chromatography method, and the respiratory enzyme activity was tested by MTT assay method. RESULTS： Compared with normal control group, in IBS rat model group intestinal mucosa Na^＋-K^＋-ATPase （22.44 ± 5.54 vs 14.20 ±3.03, P 〈 0.01）, and Ca^2＋-Mg^2＋-ATPase （16.46 ± 1.86 vs 10.63 ±1.78, P 〈 0.01） were significantly de- creased, and ATP content was also significantly lower （0.96 ± 0.18 vs 0.48 ± 0.20, P 〈 0.01）, ac companied by the respiratory enzyme activity changes （0.50 ± 0.07 vs 0.21 ± 0.05, P 〈 0.01）. CONCLUSION： Lower ATPase activity in IBS rat ileocecal mucosa is relevant to lower energy metabolism, which may cause damage to muco-sal barrier function. The results have significance on the IBS pathogenesis and treatment.

关 键 词：NA^+-K^+-ATP酶 CA^2+-MG^2+-ATP酶 肠易激综合征 回盲部 黏膜 

分 类 号：R574[医药卫生—消化系统]