白细胞介素2促进宫颈癌细胞系HeLaS3免疫球蛋白G的表达  

Interleukin 2 promotes the expression of immunoglobulin G in a cervical cancer cell line HeLaS3

作　　者：卢恬[1] 朱晓辉[1] 柳世庆[1] 郑杰[1] 邱晓彦[1] 

机构地区：[1]北京大学基础医学院免疫学系北京大学人类疾病基因研究中心,北京100191

出　　处：《北京大学学报（医学版）》2009年第2期158-161,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30371609;30572094)资助~~

摘　　要：目的:探讨白细胞介素2(interleukin2,IL-2)对宫颈癌细胞系HeLaS3表达免疫球蛋白G(immunoglobulin G,IgG)的影响。方法:用免疫荧光-流式细胞术(flow cytometry,FCM)检测IL-2刺激后HeLaS3胞膜和胞内IgG表达变化;用Western blot检测IL-2刺激HeLaS3后IgG的表达变化;用同时能扩增IgG四个亚类的引物,以RT-PCR法,从mRNA水平检测IL-2刺激后,HeLaS3γ链转录水平的变化。结果:免疫荧光-FCM结果显示,IL-2刺激48h后HeLaS3胞膜和胞内IgG表达明显上调;Western blot结果显示IL-2刺激48h后HeLaS3胞内IgG表达水平明显上调;RT-PCR显示IL-2刺激48h后HeLaS3γ链转录水平有所上升。结论:IL-2能促进HeLaS3IgG的表达。Objective：To analyze the effect of interleukin 2 （IL-2） on immunoglobulin G （IgG） expression in cervical cancer cell line HeLaS3. Methods： By immunofluorescence-flow cytometry（FCM） , we detected membrane and cytoplasmic IgG expression variation of HeLaS3 after IL-2 stimulation. By western blot, we detected IgG expression variation of HeLaS3 after IL-2 stimulation. Meanwhile, by RT- PCR with primers designed to amplify four subclasses of IgG, we detected γ transcript variation in HeLaS3 after IL-2 stimulation at mRNA level. Results： The result of immunofluorescence-FACS showed that after IL-2 stimulation for 48 hours, membrane and cytoplasmic IgG expression elevated in HeLaS3. The result of Western blot showed after IL-2 stimulation for 48 hours, IgG expression elevated in HeLaS3. Meanwhile, the result of RT-PCR showed that T1 transcript mildly elevated after IL-2 stimulation for 48 hours. Conclusion： IL-2 can promote IgG expression in cervical cancer cell line HeLaS3.

关 键 词：白细胞介素2 宫颈肿瘤 免疫球蛋白G 细胞系 肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]