淋巴瘤细胞EL-4培养上清液对CD4＋CD25＋调节性T细胞比例的调节作用  

Up-regulate of the proportion of CD4＋CD25＋ regulatory T cells by lymphoma cell EL-4 supernatants

作　　者：程育胜[1] 张帆[1] 王桂琴[1] 

机构地区：[1]山西医科大学微生物与免疫教研室,太原030001

出　　处：《肿瘤研究与临床》2009年第4期239-241,共3页Cancer Research and Clinic

基　　金：基金项目：山西医科大学科技创新基金（01200712）;山西医科大学博士启动基金（03200705）

摘　　要：目的探讨肿瘤细胞是否能上调CD4＋CD25＋调节性T细胞（Treg细胞）的比例。方法将小鼠淋巴瘤细胞株EL4培养上清液与正常小鼠脾脏淋巴细胞混合培养72h，流式细胞仪检测其中CD4＋CD25＋Treg细胞含量，RT—PCR检测Foxp3mRNA的表达，实验重复3次。结果和EL-4培养上清液混合培养的正常小鼠脾脏淋巴细胞中CD4＋CD25＋Treg细胞的比例高于对照组（P〈0．05），在CD3单抗刺激的同时加入EL-4培养上清液后，小鼠脾脏中CD4＋CD25＋Treg细胞仍呈同步增加（P〈0．05）；且Foxp3mRNA的表达增加。结论淋巴瘤细胞分泌的免疫调节因子能诱导CD4＋CD25＋Treg细胞的增生，提示肿瘤可以上调CD4＋CD25＋Treg细胞比例。Objective To explore if the tumor cell can up-regulate the proliferation ofCD4＋CD25＋ Treg cells. Methods Lymphoma cell EL-4 supernatant was combined with the normal mise spleen lymphocyte. After cultured 72 hours, CD4＋CD25＋ subset were analyzed by flow cytometry and the gene expression level of the specific marker Foxp3 were tested by RT-PCR. The experiment repeated three times. Results The supernatant derived from EL-4 can increase the proportion of CD4＋CD25＋ Treg ceils in normal mouse spleen lymphocytes than that of the control group. Moreover, when added the CD3 McAb with EL-d supernatant together, the number of CD4＋CD25＋ Treg cells increased. The level of corresponding Foxp3 mRNA also increased. Conclusion These data suggested that in the supematant may occur immune regulatory factors which up-regulated the number of CD4＋CD25＋ Treg cells and indicated that the tumorigenesis can facilitate proliferation of CD4＋CD25＋ Treg cells.

关 键 词：细胞系 肿瘤 T淋巴细胞 调节性 FOXP3 mRNA 

分 类 号：R733.1[医药卫生—肿瘤]