新疆野生啤酒花遗传多样性的形态和SSR比较分析  

The Comparison of Morphological and SSR Markers on Genetic Diversity of Wild Hops(Humulus Lupulus L.)in Xinjiang

作　　者：刘海英[1] 张霞[1] 原俊凤[1] 王仿[1] 王吉[1] 王绍明[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《北方园艺》2009年第4期15-19,共5页Northern Horticulture

基　　金：教育部"春晖计划"资助项目(Z2004-2-65043);教育部科学技术研究重点资助项目(03161)

摘　　要：采用SSR分子标记、结合形态指标对新疆野生啤酒花资源的遗传多样性进行了研究。结果表明:新疆野生啤酒花产量分别与百花重、单蔓果穗数和最长节间长3个性状呈极显著正相关;与单果穗重、主蔓长、果穗长3个性状呈显著正相关;以上6个性状具有相同的变化趋势,是影响新疆野生啤酒花产量的主要因素。根据形态学指标聚类分析,将材料分为三大类。在分子标记中,从7对引物中选出3对反应稳定、具有多态性的引物用于群体遗传多样性检测分析,得出7个居群的平均遗传多样性是A为1.5000,Ne为1.2910,h为0.1741,I为0.2597。7个居群的遗传距离在0.0197～0.3152。利用UPGMA法对7个居群间的遗传关系进行了聚类分析,分为两大类,可见新疆野生啤酒花遗传多态性较为丰富。形态学指标和遗传多样性指标的相关分析表明:全生育期、果穗结实率、叶分裂类型、叶主裂数、叶长、主蔓果穗数与遗传多样性指标具有一定的相关性。SSR and morphological analysis were carried out on wild hop in Xinjiang. The result showed that there were significant or very significany orrelation between yield and 100 cone weight, number of cones per vine, the longest internode length, average cone eight, the height of main vine ,the length of cone. They varied at the same trend. Above the six traits were essential ingredient in affecting yield. Wild hops of Xinjiang were clustered to three groups by morphologi- cal analysis. In addition, they were analyzed by using SSR method. Three pairs primers,with producing polymorphic and informative bands,were screened from seven primers. In the whole populations,average number of alleles A and effective number of alleles（Ne） were 1. 500 and 1. 2910 respectively. Nei diversity index（h） was 0. 1741. The mean of Shannon＇s index ation was 0. 2597. The genetic distance varied from 0. 0197 to 0. 3152. By cluster analysis based on SSR markers u- sing UPGMA,seven populations were divided into 2 major groups. It showed that there was higher genetic divesity in wild hop of Xinjiang. By the correlation analysis of morphological characters and genetic diversity index. It was found that the whole growing period,the seed rate, the type number of leave cleavage, the main number of leave cleavage,length of leave,number of cones pervine were related to the genetic divesity index.

关 键 词：新疆野生啤酒花 SSR 形态标记 遗传多样性 

分 类 号：S510[农业科学—作物学]