洛优7号欧李休眠期不同部位POD、CAT、SOD同功酶分析  

The Isozymes Analyse about Peroxi-dase,Catalase,Superpxide Dismutase in Different Parts of Luoyou7 Dormant Bunge Cherry

作　　者：陈苏丹[1] 李学强[1] 李秀珍[1] 程慧芳[1] 

机构地区：[1]河南科技大学林学院,河南洛阳471003

出　　处：《北方园艺》2009年第4期88-90,共3页Northern Horticulture

基　　金：河南省科技攻关资助项目(0624090004-2)

摘　　要：以生长良好、无病虫害侵染的休眠期洛优7号欧李植株为试材,采用垂直板不连续聚丙烯酰胺凝胶电泳技术,对休眠期欧李植株细根、粗根、一次枝、二次枝4个不同部位韧皮部的POD、CAT和SOD三种酶的同功酶谱带进行分析比较。结果表明:4个部位的韧皮部一共电泳出7条POD和7条SOD同功酶谱带,这两种同功酶谱带在数量和强弱上存在着明显的差异;四个部位的韧皮部一共电泳出4条CAT同功酶谱带,此同功酶谱带在数量和强弱上没有任何的差异。休眠期洛优7号欧李植株POD和SOD同功酶谱带的表达在不同的部位具有差异性,CAT同功酶谱带的表达在不同的部位没有差异性。This experiment took vigorous luoyou7 dormant bunge cherry as the material. Three isozyme bands of the phloem of radicula, tap root, shoot, twig were analysed with polylamide gel electrophoresis technique. The result showed that： Seven POD and SOD isozyme bands appeared in the phloem of four different parts on the gel. POD and SOD isozyme bands of four different parts were obviously different. The quantity and intensity of POD and SOD isozyme bands were different. Four CAT isozyme bands appeared in the phloem of four different parts on the gel. The CAT isozyme bands of four corresponding parts didn＇t have any difference in amount and the intensity. The conclusion：the expression of isozyrne bands of POD and SOD of luoyou7 dormant bunge cherry＇s different parts had an obvious difference, the CAT didn＇t have the obvious difference.

关 键 词：休眠 欧李 不同部位 过氧化物酶 过氧化氢酶 超氧化物歧化酶 

分 类 号：S662.3[农业科学—果树学]