淋巴管标记物LYVE-1、D2-40在胃癌组织中的表达及临床意义  

The expressions and significances of LYVE-1 and D2-40 in gastric cancer

作　　者：陶立生[1] 张振玉[1] 罗新华[1] 王劲松[1] 张予蜀[1] 汪志兵[1] 

机构地区：[1]南京医科大学附属南京第一医院消化科,210006

出　　处：《实用医学杂志》2009年第8期1214-1216,共3页The Journal of Practical Medicine

摘　　要：目的:检测两种新的淋巴管特异标记物LYVE-1、D2-40在胃癌组织中的表达,探讨它们在胃癌中的临床病理意义。方法:应用免疫组化对50例胃癌组织和30例正常胃组织中LYVE-1及D2-40的表达进行检测。结果:LYVE-1和D2-40在淋巴管内皮细胞阳性表达,呈棕黄色。胃癌组织中LYVE-1和D2-40阳性率均显著高于正常胃组织;癌周LYVE-1和D2-40阳性率均明显高于癌内组织;低未分化,Ⅲ～Ⅳ期,有淋巴结转移和远处转移的胃癌组织中LYVE-1和D2-40的表达水平分别比高中分化,Ⅰ～Ⅱ期,无淋巴结转移和远处转移的高。且胃癌组织各分期,各分级,无论有无远处转移和淋巴结转移及正常组织,同等条件下D2-40阳性率均高于LYVE-1,且两者表达呈显著正相关(r=0.497,P<0.05)。结论:胃癌的新生淋巴管主要位于瘤周,从而促进其淋巴结转移,且淋巴管密度与肿瘤进展程度相关。与LYVE-1相比,D2-40能更敏感和特异地预测肿瘤的淋巴管微转移,具有更好的临床病理应用价值。Objective To investigate the expressions and significances of LYVE-1 and D2-40 （new lymphatic endothelial markers） in gastric cancer. Methods Immunohistochemistry was used to detect the expressions of LYVE-1 and D2-40 in the cancer tissues from 50 cases of gastric cancer and the gastric tissues from 30 normal controls. Results LYVE-1 and D2-40 were positively expressed with brown-yellow color in the endothelial cells of lymphatic vessels. The positive rates of LYVE-1 and D2-40 were significantly higher in gastric cancer than in normal gastric tissue, while they were markedly higher in the peritumoral tissue than in the intratumoral tissue ; the expressions of LYVE- 1 and D2-40 were higher in poor differentiation, stage Ⅲ- Ⅳ and lymphatic or distant metastasis than in well and moderate differentiation, stage Ⅰ - Ⅱ , and no lymphatic or distant metastasis. The positive rate of D2-40 was higher than those of LYVE-1 in gastric cancer with or without lymphatic or distant metastasis and in normal gastric tissues. Expression of LYVE-1 was positively related to D2-40 （r = 0.497,P 〈 0.05）. Condusions The newly formed lymphatic vessels are mainly located at the peritumoral tissues, resulting in lymphatic metastasis. Lymphatic vessel density is associated with the neoplastic progression. D2-40, as compared with LYVE- 1, is more sensitive and specific in predicting lymphatic micrometastasis and thus has a greater clinical value.

关 键 词：胃肿瘤 淋巴结转移 LYVE-1 D2—40 

分 类 号：R735.2[医药卫生—肿瘤]