酶催化分光光度法测定过氧化氢  

Enzyme Catalytic Spectrophotometric Determination of Hydrogen Peroxide

作　　者：陈亚红[1] 刘红梅 田丰收[3] 宋毛平[3] 陈鹏丽[1] 

机构地区：[1]周口师范学院化学系,周口466000 [2]周口市第一高级中学,周口466000 [3]郑州大学化学系,郑州450052

出　　处：《理化检验（化学分册）》2009年第4期401-403,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省教育厅自然科学基金资助项目(No.2007150048和No.2008A150029)

摘　　要：过氧化氢能够氧化茜素红使之褪色，而模拟酶-血红蛋白对其具有催化作用。建立了一种以茜素红为指示剂的过氧化氢-茜素红-血红蛋白酶催化反应体系测定痕量过氧化氢的方法。确定了反应的最佳条件，体系的酸度为pH9．8的氨水-氯化铵缓冲溶液，最大吸收波长为525nm。线性范围为3．0×10^-7～8．0×10^-5mol·L^-1，检出限（3s／k）为5．2×10^-8mol·L^-1，表观摩尔吸光率为1．1×10。L·mol^-1·cm^-1。该方法可用于雨水及消毒水中过氧化氢含量的测定。并以此样品为基体，测定了方法的平均回收率和相对标准偏差（n=5）依次为100．39／5及3．26％。It was found that the oxidation of alizarin red by hydrogen peroxide was catalyzed enzymatlcally by haemoglobin in an ammoniacal buffer solution of pH 9. 8, leading to decoloration of alizarin red. Absorption maximum of the reaction system was found at the wavelength of 525 nm. Linear relationship was kept in the range of 3.0×10^-7--8.0×10^-5mol·L^-1 between values of decrease in absorbance （△A） and concentration of hydrogen peroxide. Apparent molar absorptivity （ε525） and detection limit （3s/k） were found to be 1. 1×10^4L·mol^-1·cm^-1 and 5.2×10^-8 mol·L^-1 respectively. Base on these facts, the method mentioned above was proposed for determination of trace amounts of hydrogen peroxide. Tests for average recovery and precision of the method were peroformed on the base of samples of rainwater and disinfactant liquors, average values of recovery and RSDts （n= 5） obtained were 100. 3G and 3. 26% respectively.

关 键 词：分光光度法 酶催化 过氧化氢 血红蛋白 茜素红 

分 类 号：O657.32[理学—分析化学]