用卵巢癌可溶性抗原体外诱导产生具有肿瘤选择性细胞毒T细胞  

Experimental studies on the cytotoxic T lymphocytes induced by soluble antigen of ovarian carcinoma.

作　　者：张庆波[1] 张淑杰[1] 边洪荣[1] 石卫[2] 李艳芬[2] 万云霞[2] 翟红[2] 陈毓仙[2] 

机构地区：[1]华北煤炭医学院,063000 [2]中国医学科学院中国协和医科大学肿瘤研究所

出　　处：《现代妇产科进展》1998年第1期8-10,共3页Progress in Obstetrics and Gynecology

摘　　要：目的：提高治疗卵巢癌的疗效，探讨新的肿瘤生物治疗方法。方法：提取卵巢癌细胞可溶性抗原，用此抗原作细胞免疫体外实验。结果：用卵巢癌可溶性抗原和抗CD3单抗体外诱导产生了具有肿瘤选择性的细胞毒性T细胞，这种细胞体外增殖快，对抗原来源的肿瘤细胞具有选择性杀伤作用，细胞表型分析CD8＋细胞占90％以上。结论；建立了一种新的肿瘤生物治疗方法，并为肿瘤生物治疗和肿瘤疫苗的研制提供了实验基础。To study on enhancement of the effect of biotherapy to ovarian carcinoma: A new method for treatment of cancer. Methods: Tumor soluble antigen (TSA )was extracted. In vitro experiments of cellular immunology were done with the TSA. Results:Peripheral blood mononuclear cells(PBMC)were induced by the TSA and anti-CD3 monoclonal antibody (CD3-McAb) to develop into cytotoxic T lymphocyte (CTL ), which proliferated rapidly in vitro and had a high cytotoxicity to the tumor cells came from TSA. Analysis of phenotype of the CTL showed that CD8+, CTL were more than 90%.Conclusion: A new kind of method has been developed, which provides an experimental basis for the development of tumor vaccine and the biotherapy to tumor.

关 键 词：卵巢肿瘤 免疫疗法 CTL 可溶性抗原 诱导 细胞毒 

分 类 号：R737.310.5[医药卫生—肿瘤]