大鼠全脑缺血再灌注损伤后海马自噬相关蛋白Beclin 1的表达变化  

Expression of autophagy-associated protein Beclin 1 in the hippocampus after global brain ischemia-reperfusion injury in rats

作　　者：强强[1] 马建芳[2] 王晓强[1] 潘静[2] 赵开军[1] 孙青芳[1] 卞留贯[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院神经外科,上海200025 [2]上海交通大学医学院附属瑞金医院神经内科,上海200025

出　　处：《中国微侵袭神经外科杂志》2009年第4期180-182,共3页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：上海市科委重点课题资助项目(编号:06JC14052);上海市自然科学基金资助课题(编号:08ZR1413800)

摘　　要：目的研究自噬相关蛋白Beclin1在大鼠全脑缺血-再灌注损伤后海马中的表达情况,并探讨其意义。方法使用四动脉结扎法制作大鼠全脑缺血-再灌注损伤模型,实验动物随机分为:假手术组和缺血再灌注组。在全脑缺血15min后,分别再灌注0min、30min、3h、6h、12h、24h、1d、3d,使用Western blot检测各个时间点大鼠全脑缺血-再灌后海马自噬相关蛋白Beclin1的表达情况。结果与假手术组比较,大鼠全脑缺血-再灌注损伤后1d,海马区Beclin1蛋白的表达最强(P<0.05)。结论大鼠全脑缺血-再灌注损伤后海马自噬相关蛋白Beclin1表达上调,表明脑缺血-再灌注损伤后海马区域的自噬活性上调。Objective To study the expression of autophagy-associated protein Beclin 1 and its significance in the hippocampus after global cerebral ischemia-reperfusion injury in rats. Methods The global cerebral isehemia was induced by four-vessel occlusion in Sprague-Dawley （SD） rats. The rats were randomly divided into sham-operated group and ischemia-reperfusion group. Fifteen minutes after the cerebral isehemia, the reperfusion was performed at 0 and 30 min, 3, 6, 12 and 24 h, and 1 and 3 d. The expression ofBeclin 1 in the hippocampus after ischemia-reperfusion was detected by immunoblotting analysis at each time point. Results Compared with the sham-operated group, the expression level of Beclin 1 reached a peak at 1 d after cerebral ischemia-reperfusion （P〈0.05）. Conclusion Global cerebral ischemiareperfusion injury induces up-regulation of Beclin 1 in rat hippocampus, which demonstrates up-regulation of autophagic activity in rat hippocampus after cerebral ischemia and reperfusion.

关 键 词：再灌注损伤 海马 自噬 

分 类 号：R743[医药卫生—神经病学与精神病学]