鱼藤酮对大鼠纹状体谷氨酸-谷氨酰胺环路的影响  

Effects of Rotenone on Glutamate-glutamine Circulation in Rats Striatum

作　　者：刘辉[1,2] 郭魁亮[1] 吴强[2] 董兆君[2] 

机构地区：[1]白求恩军医学院卫勤教研室,河北石家庄050081 [2]第三军医大学毒理学教研室,重庆400038

出　　处：《环境与职业医学》2009年第2期159-161,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:30400347)

摘　　要：[目的]观察鱼藤酮(rotenone)对大鼠纹状体谷氨酸-谷氨酰胺环路代谢的影响。[方法]将24只SD大鼠随机分为正常对照组、溶剂对照组、2.0mg/kg和4.0mg/kg鱼藤酮染毒组。采用高效液相色谱法(HPLC)检测鱼藤酮染毒大鼠纹状体谷氨酸(glutamate,Glu)和谷氨酰胺(glutamine,Gln)含量;使用逆转录聚合酶链式反应(RT-PCR)法观察谷氨酰胺合成酶(glutamine synthetase,GS)基因表达的变化;采用分光光度法检测谷氨酰胺酶(phosphate activated glutaminase,PAG)与GS活性。[结果]与正常对照组比较,4.0mg/kg染毒组大鼠纹状体Glu含量明显升高(P<0.01),而Gln含量明显降低(P<0.01);4.0mg/kg染毒组GS mRNA表达明显增强(P<0.05);4.0mg/kg染毒组GS活性却明显降低,PAG活性明显升高(P<0.01)。[结论]鱼藤酮诱导大鼠纹状体Glu含量明显升高,可能是导致中脑神经元兴奋性损伤的的主要原因之一。[Objective] To study the effects of rotenone on glutamate-glutamine circulation in rats striatum. [ Methods ] 24 Sprague-Dawley rats were divided into four groups randomly： the control group, solvent control group, 2.0 mg/kg and 4.0 mg/kg rotenone treated groups. The concentration of glutamate and glutamine in rats striatum were assayed by high performance liquid chromatography （ HPLC ）. The expression of glutamine synthetase （ GS ）mRNA was measured by RT-PCR, and the activities of GS and phosphate activated glutaminase（ PAG ）were determined by using GS and PAG detect kit. [ Results ] Compared with control group, the release of glutamate in rats striatum increased （P 〈 0.01 ）and the concentration of glutamine decreased （P 〈 0.01 ）, the activity of GS declined, but PAG activity elevated obviously in 4.0 mg/kg rotenone treated group. [ Conclusion ] Glutamate-glutamine circulation was damaged in the brain of rotenone treated rats, which may be responsible for neuron degeneration induced by rotenone.

关 键 词：鱼藤酮 谷氨酸 谷氨酰胺 谷氨酰胺合成酶 谷氨酰胺酶 

分 类 号：R114[医药卫生—卫生毒理学]