封闭群比格犬遗传标志微卫星引物的筛选  

Screening of Microsatellite Primers Used for Genetic Mark of Closed Colony in Beagle Dogs

作　　者：孙兆增[1] 曾林[1] 赵太云[1] 孔德强[1] 洪宝庆[1] 胡仲明[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071

出　　处：《实验动物与比较医学》2009年第2期105-108,共4页Laboratory Animal and Comparative Medicine

基　　金：军事医学科学院创新基金

摘　　要：目的筛选出有效的微卫星引物，利用微卫星遗传标志对封闭群内比格犬的个体遗传情况进行分析。方法从文献中筛选杂合度在0．4～0．8的微卫星引物24对，对本场英系比格犬封闭群中随机筛选出的12只比格犬的混合基因组进行了扩增。结果24对引物中，有7对微卫星引物的扩增结果具有较好的多态性和重复性。利用这7对引物，对8只玛斯公司美系比格犬的混合基因组进行了扩增，结果与本场英系比格犬的扩增结果具有明显的差异。又利用这7对引物对随机筛选出的6只比格犬的个体遗传情况进行了分析，其中3只比格犬表现出明显的差异。结论作者筛选的7对微卫星引物可以对本场比格犬种群的个体遗传情况进行初步分析。Objective To select the effective microsatellite primers and analyze the genetic circumstance of individual Beagle dogs. Methods Twenty-four pair of primers with the heterozygotic degree between 0.4-0.8 were selected from the literature. The primers were authenticated used the mix-genome of 10 Beagle dogs. Results Among the 24 pair primers, 7 pair of primers were polymorphism and reproducibility. The 7 pair of primers used the mix-genome of 10 Beagle dogs of MaSi corporation were also tested, The results showed obviously different from Beagle dogs of our plant. The genetic circumstance of 6 individual Beagle dogs were also analyzed used the same 7 pair primers, four of them showed obviously different. Conclusion The 7 pair primers were suitable for analyzed the genetic circumstance of individual Beagle dogs primarily.

关 键 词：比格犬 封闭群 微卫星 

分 类 号：Q95-33[生物学—动物学]