基于BioMEMS微柱矩阵的细胞牵引力测量(英文)  

Cell traction forces measurement based on BioMEMS microposts matrix

作　　者：周治国[1] 刘志文[1] 范哲意[1] 

机构地区：[1]北京理工大学信息与电子学院,北京100081

出　　处：《医用生物力学》2009年第2期112-117,共6页Journal of Medical Biomechanics

摘　　要：本文综述了基于生物微机电系统(BioMEMS)微柱矩阵的细胞牵引力测量方法。细胞牵引力对于许多生物学过程非常关键,决定着许多细胞功能,包括细胞迁移、细胞外基质构建、信号传导等。细胞通过纳牛顿量级的牵引力使细胞外基质局部变形来探测其机械顺从性。精确测量细胞牵引力大小及分布对细胞生物学、组织工程等生物医学研究具有重要意义。BioMEMS的发展使高深宽比聚二甲基硅氧烷(PDMS)微柱矩阵被开发出来作为传感器用来探测细胞纳牛力学及在体外研究细胞的机械性质。细胞贴附在微柱矩阵顶端,并且在多个柱顶端间延展迁移,这个过程会造成微柱发生如垂直悬臂梁般的弯曲形变。采用这种致密、垂直、离散微柱矩阵结构替代传统测量的连续介质,通过对微柱形变的显微图像处理,细胞牵引力可以被直接定性定量测量,精度可以达到数十nN/μm量级。首先简要介绍了传统细胞牵引力测量方法,接下来着重于论述基于BioMEMS微柱矩阵的测量方法,阐述了其原理、制作工艺、表面处理及细胞实验等。最后对微柱矩阵结构的坍塌问题进行了讨论。A review of cell traction forces （CTFs） measurement based on Biological Micro Electromechanical Systems （BioMEMS） microposts matrix is presented. CTFs are exerted by cells and transmitted to the underlying substrate through focal adhesions and close contacts, which is essential for cells movement. Cells probe the mechanical compliance of the exb＇acellular matrix （ECM） in part by locally deforming it with nanonewtonscale traction forces. Precision measurement of CTFs is significant for many researches such as cell biology and tissue engineering and so on. Enabled by the advancement in BioMEMS technology, surface treated high aspect ratio Polydimethyisiloxane （PDMS） microposts matrix devices, which serve as BioMEMS sensors for detecting cellular nanoforces and studying in vitro cell mechanics, have been developed. Closely spaced vertical microposts matrixes were designed to encourage cells to attach and spread across multiple microposts, and to bend the microposts like vertical cantilevers as the cells Iocomote on the surface. Using this dense and discrete matrix of microposts rather than a conventional continuous substrate, CTFs can be directly measured and quantified by processing the microscopy images of the deformations of microposts. The resolution of the force was in tens of nN/μm scale. At first, the conventional CTFs measurement methods were concisely summarized. Then BioMEMS microposts matrix method was described in detail, including principle and fabrication process, surface treatment and cell experiment results. Furthermore, high aspect ratio structure collapse problem was investigated.

关 键 词：细胞牵引力 生物微机电系统 PDMS微柱矩阵 

分 类 号：R318.01[医药卫生—生物医学工程]