胃癌患者外周血人端粒酶逆转录酶mRNA表达及其临床意义  

Expression of Human Telomerase Reverse Transcriptase(hTERT) mRNA in Peripheral Blood with Gastric Cancer and Its Clinical Significance

作　　者：刘永萍[1,2] 凌扬[1,2] 王风军[1] 孔颖泽[2] 张亚平[1,2] 盛桂风[2] 徐建忠[2] 杨全良[2] 

机构地区：[1]苏州大学附属常州肿瘤医院临床肿瘤学实验室,213001 [2]苏州大学附属常州肿瘤医院肿瘤内科,213001

出　　处：《肿瘤防治研究》2009年第4期302-305,共4页Cancer Research on Prevention and Treatment

基　　金：江苏省卫生厅面上科研课题资助项目(H200553);江苏省常州市科技计划资助项目(C2005215)

摘　　要：目的研究胃癌患者外周血人端粒酶逆转录酶(hTERT)mRNA的表达,并探讨其临床意义。方法应用Taq Man实时定量RT-PCR法检测58例患者、20名健康对照者外周血hTERT mRNA的表达,随访2年。结果胃癌患者外周血hTERT mRNA阳性率为55.17%,显著高于健康对照组(P<0.001);hTERT mRNA表达与肿瘤浸润深度、淋巴结转移、远处转移和临床分期相关,其中淋巴结转移和远处转移是影响hTERT mRNA表达的独立因素;近期疗效无效的患者hTERT mRNA的阳性率显著高于近期疗效有效的患者(P=0.003)。化疗前hTERT mRNA阳性与阴性患者的1年生存率分别是28.13%和76.92%。结论外周血hTERT mRNA可作为检测胃癌患者微转移的分子标志,定期监测有助于评估化疗疗效和预测预后。淋巴结转移和远处转移与hTERT mRNA表达密切相关。Objective To investigate the expression of human telomerase reverse transcriptase （hTERT） mRNA in peripheral blood of patients with gastric cancer and to discuss its clinical significance. Methods hTERT mRNA in peripheral blood was detected by TaqMan real time quantitative reverse transcriptase-polymerase chain reaction（RT -PCR）in 58 patients with gastric cancer and 20 healthy subjects. The patients were followed for 2 years. Results The positive rate of hTERT mRNA in patients with gastric cancer was 55. 17% （ 32 / 58）,which was significantly higher than that in the healthy controls（P〈 0. 001）. Univariate analysis revealed that depth of tumor invasion（P = 0. 038）, tumor lymphatic metastasis（P = 0. 008） ,distant metastasis （P〈0. 001 ）and clinical stages （P = 0. 003）were related to the hTERT mRNA expression in peripheral blood of the gastric cancer patients,among them lymphatic metastasis and distant metastasis were independent compact factors of the hTERT mRNA expression in peripheral blood of gastric cancer patients. The positive rate of hTERT mRNA in patients with chemotherapy no-response was significantly higher than those in the patients with chemotherapy response. The rate of one year survival for patiens with positive hTERT mRNA before chemotherapy was lower than those with negative one （P〈0. 001）. Conclusion hTERT mRNA can be used as a marker for the detection of tumor micrometastasis in gastric cancer, and it may be important to regularly detection for evaluating chemotherapy response and prognosis. Lymphatic metastasis and distant metastasis were closely correlated with the ex pression of hTERT mRNA in peripheral blood of patients with gastric cancer.

关 键 词：胃肿瘤 人端粒酶 逆转录酶 疗效 预后 

分 类 号：R735.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]