药物抑制兔眼晶体囊膜上皮细胞增生效果评价  

Inhibition effect of drugs on proliferation of rabbit lens epithelial cells

作　　者：陈大本[1,2] 李涤臣[1,2] 马进[1,2] 王剑峰[1,2] 

机构地区：[1]蚌埠医学院附属医院眼科 [2]蚌埠医学院病理学教研室

出　　处：《蚌埠医学院学报》1998年第2期76-77,共2页Journal of Bengbu Medical College

摘　　要：目的：探索细胞生长抑制药物预防后囊混浊（ＰＣＯ）的可能性。方法：（１）晶体后囊膜上皮细胞（ＲＬＥＣ）体外培养，传代在２４孔板内用不同浓度的氟脲嘧啶（５Ｆｕ）、阿霉素（ＡＤＭ）培养２４ｈ、７２ｈ做活细胞计数，求出半效抑制量（ＩＤ５０）；（２）兔眼晶体囊外摘除（ＥＣＣＥ）加人工晶体（ＩＯＬ）植入术后，连续应用５Ｆｕ（５ｍｇ／ｄ）、ＡＤＭ（０．０５ｍｇ／ｄ）５次，于３０ｄ、７５ｄ分别取出残存囊膜和其余眼球组织，作ＨＥ染色、溶菌酶和抗胰蛋白酶（ａｌｐｈａ１ａｎｔｉｔｒｙｐｓｉｎ，ＡＡＴ）免疫组化标记。结果：（１）５Ｆｕ、ＡＤＭ用于传代培养ＲＬＥＣ２４ｈＩＤ５０分别为０．６２μｇ／ｍｌ、４．８９ｎｇ／ｍｌ；７２ｈ０．３８μｇ／ｍｌ、４．０５ｎｇ／ｍｌ。（２）两实验组３０ｄ囊膜上皮均有小灶性增生；７５ｄ有小灶性增生或增生不明显。对照组囊膜上皮细胞３０ｄ、７５ｄ均有向纤维母细胞样细胞移行。结论：５Ｆｕ、ＡＤＭ实验室生长抑制试验与兔眼结膜下注射。Objective:To study the inhibition effect of 5fluorouracil(5Fu) and adriamycin(ADM) on proliferation of rabbit lens epithelial cells(RLEC) and prevention of opacification of posterior capsulae(PCO).Methods:(1)Cultivated RLEC in vitro in 24well tissue culture plates and exposed them to 5Fu and ADM in different concentratios for 24 and 72 hours.The number of vital cells were counted and their ID50 calculated.(2)5Fu and ADM were used on eyes performed with ECCE and IOL implantation for five times.On the 30th and 75th day,the remaining part of capsulae and eye tissue were taking for HE and immunohistochemical staining.Results:(1)ID 50 of 5Fu and ADM exposed to RLEC for 24 hours were 0.62 μg/ml,4.89 ng/ml,and for 72 hours were 0.38 μg/ml,4.05 ng/ml respectively.(2)Small area of proliferation of RLEC was discovered during 30 days and 75 days cultivation in these two experimental groups.In control group,RLEC transformed into fibroblasts.Conclusions:5Fu and ADM used in vitro or injected subconjunctivally,show similar effect of inhibition on RLEC.

关 键 词：后发性 后囊混浊 白内障 预防 晶体上皮细胞 

分 类 号：R776.101[医药卫生—眼科]