美托洛尔对大鼠冠状动脉微栓塞后心肌细胞凋亡及caspase-12活化的影响及意义  

Effects of metoprolol on cardiomyocyte apoptosis and caspase-12 activation after coronary microembolization in rats

作　　者：朱汉华[1] 李浪[1] 汪熠[1] 陆永光[1] 赵献明[1] 文伟明[1] 

机构地区：[1]广西医科大学第一附属医院心内科,广西心血管病研究所,广西南宁530021

出　　处：《中国病理生理杂志》2009年第4期642-646,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.C030313);广西研究生教育创新计划资助项目(No.2006105981002M08)

摘　　要：目的:探讨美托洛尔对大鼠冠状动脉微栓塞后心肌细胞凋亡及caspase-12活化的影响及意义。方法:30只大鼠随机分为假手术组、微栓塞组、美托洛尔组(每组n=10),经左室注入42μm微栓塞球,建立大鼠冠状动脉微栓塞模型,假手术组注射生理盐水代替微栓塞球,美托洛尔组为微栓塞术前30 min静脉注射美托洛尔。各组术后6 h分别心脏超声检测左室射血分数(LVEF),TUNEL检测心肌细胞凋亡,Western blotting检测凋亡蛋白caspase-12的活化。结果:①与假手术组比较,微栓塞组LVEF显著下降(P<0.05);与微栓塞组比较,美托洛尔组LVEF没有显著差异。②与假手术组比较,微栓塞组心肌细胞凋亡率、活化的caspase-12含量显著增加(均P<0.05);与微栓塞组比较,美托洛尔组心肌细胞凋亡率、活化的caspase-12含量显著减少(均P<0.05)。结论:美托洛尔抑制大鼠冠状动脉微栓塞后心肌细胞凋亡及caspase-12活化。AIM： To investigate the effects of metoprolol on cardiomyocyte apoptosis and caspase - 12 activation after coronary microembolization in rats. METHODS： 30 rats were randomized to shamoperated group （S group）, coronary microembolization group （CME group） and metoprolol group. Coronary microembolization models were produced by injection of 42μm microspheres （3000/0. 1 mL） into the left ventricle during 10 seconds ascending aorta occlusion in rats. The S groups were injected saline instead. Intravenous metoprolol was infused into the rats assigned to the metoprolol groups. Cardiomyocyte apoptosis was detected with in TUNEL staining. The activation of caspase - 12 was measured by Western blotting analysis. Left ventricular ejection fraction （LVEF） was assessed by transthoracic two -dimensional echo- cardiography. RESULTS ： （~） LVEF was significantly decreased in CME group compared to S group （ P 〈 0. 05 ）. No statistical difference between the metoprolol group and CME group was observed. （2）Compared with S group, the apoptosis rate of cardiomyocytes and the levels of activated caspase - 12 proteins in CME group were significantly increased （ all P 〈 0. 05 ）. Compared with CME group, the apoptosis rate of cardiomyocyte and the levels of activated caspase - 12 proteins in metoprolol group were significantly decreased （ all P 〈 0. 05 ）. CONCLUSION ： Metoprolol inhibits the apoptosis of cardiomyocytes and the activation of caspase - 12 after coronary microembolization.

关 键 词：美托洛尔 冠状动脉微栓塞 细胞凋亡 半胱氨酸天冬氨酸蛋白酶12 

分 类 号：R363[医药卫生—病理学]