高果糖大鼠血清TG、UA、血糖的动态变化规律及机制研究  

Pathogenesis of hypertriglyceridemia with hyperuricemia and hyperglycemia induced by high fructose

作　　者：刘小青[1] 张冰[1] 林志健[1] 孔悦[1] 杨红莲[1] 庄红艳[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国病理生理杂志》2009年第4期651-655,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30472282);北京市自然科学基金资助项目(No.7052036)

摘　　要：目的:动态观察高果糖模型大鼠血清甘油三酯(TG)、尿酸(UA)、血糖(GLU)及全血3-磷酸甘油醛脱氢酶(GAPDH)活性和肝脏基因表达的变化规律,并探讨其病理机制。方法:随机将SD大鼠分为对照组、果糖组和非诺贝特治疗组。对照组予普通大鼠饲料;果糖组给予高果糖饲料[5];菲诺贝特治疗组给予高果糖饲料,同时灌胃菲诺贝特100 mg.kg-1.d-1进行给药。对照组、果糖组灌胃等量蒸馏水。实验期间动态检测血清TG、UA、GLU;改进正向反应法测定全血GAPDH活性;Quanti Gene(QG)分析法测肝GAPDH mRNA转录水平。结果:与对照组比,果糖组7-28 d TG持续明显升高;14-28 d UA明显升高;GLU仅在28 d明显升高;GAPDH活性及基因表达7-28 d均明显降低。菲诺贝特治疗组仅在7 d见明显降TG作用;而对UA则有明显升高的作用;对GLU在28d有明显的降低作用;对GAPDH活性及基因表达仅在造模7 d时有明显升高作用;其余未见明显变化。结论:①高果糖造模初期TG明显升高,随造模时间延长相继伴发UA及GLU明显升高。②TG、UA、GLU升高可能与GAPDH活性及基因表达降低有关。③菲诺贝特在高TG时有明显降TG作用,对伴发高UA和高GLU时未见明显影响。④菲诺贝特降TG的作用机制可能与提高GAPDH活性和基因表达有关。AIM： To observe the levels of triglyceridemia （TG）, uricemia （UA）, glycemia （GLU）, the activity of 3 - glyceraldehyde phosphate dehydrogenase （GAPDH） in blood and the gene expression in the liver in the animal model of hypertriglyceridemia, hyperuricemia and hyperglycemia. METHODS： SD rats were randomly divided into three groups, control group, fructose group, fructose and fenofibrate treated group. Rats in control group were fed with standard chow. Rats in fructose group were fed with high fructose diet. Rats in fructose and fenofibrate group were fed with high fructose diet, and treated with fenofibrate 100 mg·kg^-1·d^-1 by intragastric administration at the same time. Rats in control group and fructose group were given distilled water by intragastric administration. The levels of TG, UA and GLU were detected. Improved method was used to measure the activity of GAPDH. Quanti Gene technology was applied to determine the transcriptional level of GAPDH mRNA. RESULTS ： During 7 - 28 d, the level of TG in fructose group was significantly and persistently high. During 14 - 28 -d, the level of UA was higher. The level of GLU higher than that in control group was only observed at 28th day. The GAPDH activity change in blood and the expression in liver were significantly lower than that in normal during 7 - 28 d. Fenofibrate had the effect on reducing TG only at 7th day and reduced the level of GLU significantly at 28th day. Fenofibrate also increased the GAPDH activity in blood and the expression in liver at 7th day. CONCLUSION ： （1） The level of TG is significandy and persistently high in the early days by feeding with excessive fructose. The levels of UA and GLU are higher with the time cause of the model development. （2） The significantly higher level of TG, UA and GLU may be correlated with the reduction of the GAPDH activity in blood and the expression in liver. （3） Fenofibrate has the effect of reducing the TG level only in the condition of hypertriglyceridemia, but not in th

关 键 词：果糖 高甘油三酯血症 高甘油三酯并高尿酸血症 高甘油三酯并高尿酸并高血糖血症 甘油醛 3-磷酸脱氢酶类 非诺贝特 

分 类 号：R363[医药卫生—病理学]