罗勒多糖抗卵巢癌侵袭转移的体外实验研究  

Experimental observation of basil polysaccharide against the invasion and metastasis of ovarian cancer cell lines in vitro

作　　者：王婷[1,2] 郑广娟[1] 闫实[2] 曲迅[2] 

机构地区：[1]山东中医药大学病理教研室,山东济南250014 [2]山东大学齐鲁医院临床基础研究所,山东济南250012

出　　处：《中国病理生理杂志》2009年第4期661-665,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30472261No.30671902)

摘　　要：目的:探讨罗勒多糖抗卵巢癌侵袭转移的作用机制及其肿瘤乏氧微环境对其效应的影响,为临床应用研究提供依据。方法:罗勒多糖分别在常氧(21%O2、5%CO2)和乏氧(1%O2、5%CO2和94%N2)环境中作用于人卵巢癌SKOV3细胞,形态学观察不同氧环境下各组细胞形态差异;Transwell小室实验检测各组细胞的侵袭运动能力;明胶酶谱法分析各组细胞分泌的基质金属蛋白酶-2(MMP-2)活性差异;RT-PCR技术检测骨桥蛋白(OPN)mRNA表达水平;免疫细胞化学法观察各组细胞胞内OPN表达情况。结果:与对照组相比,罗勒多糖在常氧和乏氧环境下都能够降低SKOV3细胞的侵袭运动能力,抑制细胞中MMP-2的分泌,抑制人卵巢癌SKOV3细胞中OPN的表达(转录水平、蛋白水平),且乏氧环境下罗勒多糖的上述作用更为显著。结论:低氧显著增强人卵巢癌SKOV3细胞的迁移能力,罗勒多糖可能通过下调骨桥蛋白表达及基质金属蛋白酶-2的分泌,抑制其侵袭运动能力。AIM： To investigate the mechanism of basil polysaccharide in inhibiting the invasion and metastasis ability of ovarian cancer, and to evaluate the potency of hypoxia tumour microenvironment in affecting the action of basil polysaccharide. METHODS： The human ovarian cancer cell line SKOV3 was cultured with basil polysaccharide under normoxia （21% 02, 5% CO2） and hypoxia （1% 02,5% CO2,94% N2 ）, respectively. Morphology of this cell line in different groups under different oxygen concentrations was observed under inverted phase contrast microscope. The cell invasiveness was tested by transwell method and the activities of matrix metalloproteinase - 2 （ MMP - 2） among each group were analyzed by gelatin zymography. The expressions of OPN mRNA and protein levels were detected by RT - PCR and immunohistochemistry, respectively. RESULTS： Compared to the control group, basil polysaccharide decreased the invasiveness and migrating ability and inhibited the secretion of MMP - 2 in SKOV3 cells. Basil polysaccharide also reduced the expression level of osteopontin （transcriptional level and protein level） of the SKOV3 cells in both normoxic and hypoxic conditions. Moreover, these functions of basil polysaccharide were more obviously in hypoxia. CONCLUSION： The migrating ability of the SKOV3 cells is enhanced under hypoxic condition. Basil polysaccharide may inhibit the invasiveness and migrating ability of SKOV3 cells through reducing osteopontin expression and MMP - 2 secretion.

关 键 词：罗勒多糖 卵巢肿瘤 缺氧 骨桥蛋白 基质金属蛋白酶2 

分 类 号：R363[医药卫生—病理学]