巨噬细胞移动抑制因子促进肺成纤维细胞增殖和胶原合成  

Effect of recombinant MIF on lung fibroblast proliferation and collagen synthesis in vitro

作　　者：陈培芬[1] 罗雅玲[1] 赖文岩[2] 邢晓雯[2] 胡斯明[1] 

机构地区：[1]南方医科大学附属南方医院呼吸科,广东广州510515 [2]南方医科大学附属南方医院心内科实验室,广东广州510515

出　　处：《中国病理生理杂志》2009年第4期699-702,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30770936)

摘　　要：目的:观察重组巨噬细胞移动抑制因子(rMIF)对人胚肺成纤维细胞(MRC-5)的作用,探讨哮喘气道重塑的发生机制。方法:不同浓度的rMIF(25-100μg/L)分别作用于MRC-5细胞12 h、24 h或48 h,用CCK-8法测细胞增殖率,羟脯氨酸法检测细胞上清胶原水平,RT-PCR法检测Ⅰ型胶原mRNA表达,Western blot-ting检测成纤维细胞分泌Ⅰ型胶原。结果:与对照组比,50μg/L和100μg/L的rMIF刺激24 h和48 h,显著促进MRC-5增殖(P<0.05或P<0.01)。刺激48 h后,细胞培养上清中,对照组及实验组均可检测到羟脯氨酸,100μg/L rMIF显著促进羟脯氨酸分泌(P<0.01);rMIF能够剂量依赖地刺激Ⅰ型胶原mRNA和蛋白合成(P<0.05或P<0.01)。结论:rMIF通过刺激成纤维细胞增殖及胶原合成,可能在哮喘气道重塑的发病机制中发挥关键作用。AIM ： To investigate the influence and mechanism of recombinant macrophage migration inhibitory factor （rMIF） on fibroblasts. METHODS： MRC -5 fibroblasts were divided into two groups： the treated group was treated with rMIF （25 - 100 μg/L, 12 h, 24 h or 48 h） and the control was non - rMIF treatment. The activity of proliferation in both groups was investigated and compared by CCK - 8 means. Synthesis of collagen in the culture supematants was detected by the hydroxyproline. The expression of collagen type Ⅰ mRNA was examined using RT - PCR analysis. The level of collagen type Ⅰ protein induced by rMIF was quantified by Western blotting. RESULTS： The production of proliferation ratio of fibroblasts treated with 50 μg/L and 100 μg/L rMIF at 24 h or 48 h were increased obviously （ P 〈 0. 05 or P 〈 0. 01 ）. The collagen synthesis significantly increased after stimulation with 100 μg/L rMIF for48 h （P 〈0. 01 ）. rMIF significantly increased the expression of collagen type I mRNA and protein in a dose dependent manner compared with control （P 〈0. 05 or P 〈0. 01 ）. CONCLUSION： rMIF upregulates the proliferation of fibroblasts and has an effect on the production of collagen. These results suggest that MIF may play important roles in the pathogenesis of airway remodeling.

关 键 词：巨噬细胞游走抑制因子 成纤维细胞 胶原 哮喘 

分 类 号：R363[医药卫生—病理学]