互隔交链孢酚通过PKA-CREB信号通路激活NIH3T3细胞中DNA聚合酶β表达  

Alternariol induces DNA polymerase β expression via the PKA-CREB pathway in NIH3T3 cells

作　　者：赵继敏[1] 路静[1] 刘康栋[1] 王瑾瑾[1] 杨洪艳[1] 黄幼田[1] 董子明[1] 

机构地区：[1]郑州大学基础医学院病理生理教研室,河南郑州450052

出　　处：《中国病理生理杂志》2009年第4期744-748,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30471952);河南省教育厅自然科学基金资助项目(No.2007310020)

摘　　要：目的:探讨互隔交链孢酚(AOH)诱导小鼠胚胎成纤维细胞NIH3T3中DNA聚合酶β(DNA polβ)表达增高的分子信号通路。方法:①15μmol/L AOH作用NIH3T3细胞16 h,利用Western blotting方法检测细胞中DNA polβ的表达,并利用磷酸化CREB抗体检测AOH作用后NIH3T3细胞中CREB信号分子是否被激活。②利用PKA-CREB信号通路特异性抑制剂H89预处理NIH3T3细胞1 h,再加入AOH作用,分别检测磷酸化CREB和DNA polβ表达的变化。结果:①与溶剂对照组相比,AOH诱导NIH3T3细胞中DNA polβ表达明显增高,而且磷酸化CREB蛋白含量也明显增加,差异均显著。②用PKA特异性抑制剂H89预处理,可部分抑制NIH3T3细胞中CREB蛋白的磷酸化,并且降低了AOH引起的NIH3T3细胞中DNA polβ的表达增加。结论:PKA-CREB信号通路在AOH诱导的NIH3T3细胞中DNA polβ表达起重要作用。AIM： To investigate the signal transduction induced DNA polymerase β expression by alternariol （AOH） in NIH3T3 cells. METHODS： The NIH3T3 cells were treated with 15 μmol/L AOH for 16 h or the cells were pretreated with an inhibitor of PKA （H89） for 1 h, and then exposed to AOH. The changes of DNA polβ expression and phospho - CREB in the ceils with different treatments were determined by Western blotting and immunocytochemistry analysis. RESULTS ： The expression of DNA polymerase βand phospho - CREB in NIH3T3 cells induced by AOH were significantly higher than that in control group （ P 〈 0. 05 ）. However, H89 partly blocked the AOH - induced phospho - CREB and DNA polβ expression. CONCLUSION： Altemariol up - regulates DNA polymerase β expression via the PKA - CREB pathway in NIH3T3 cells.

关 键 词：链格孢酚 DNA聚合酶Β 蛋白激酶A CAMP反应元件结合蛋白 NIH3T3细胞 

分 类 号：R735.1[医药卫生—肿瘤]