低频重复经颅磁刺激预处理对匹鲁卡品致痫大鼠的影响  

The effects of pretreatment with low-frequency transcranial magnetic stimulation on rats with pilocarpine-induced seizures

作　　者：黄敏[1] 余巨明[1] 王晓明[1] 王莉[1] 

机构地区：[1]四川省南充市川北医学院附属医院神经内科,南充637000

出　　处：《中华物理医学与康复杂志》2009年第4期228-231,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：四川省科技厅攻关课题（05SCT022-016）

摘　　要：目的探讨低频经颅磁刺激（TMS）对匹鲁卡品致痫大鼠痫性发作的影响。方法共选取50只成年雄性清洁级Wistar大鼠，根据预先设定的磁刺激频率将其分为0Hz组（假刺激组）、0．3Hz组、0．5Hz组、0．8Hz组及1．0Hz组，各组大鼠经相应频率磁刺激预处理后，将其制成氯化锂一匹鲁卡品急性癫痫模型。观察各组大鼠在注射匹鲁卡品90min内痫性发作的潜伏期及痫性发作行为学表现。结果与假刺激组比较，各磁刺激组癫痫发作潜伏期均明显延长，与假刺激组间差异均有统计学意义（P〈0．05或0．01）；与0．3Hz组及1．0Hz组比较，0．5Hz组及0．8Hz组潜伏期延长更显著，组问差异具有统计学意义（P〈0．05）。与假刺激组比较，0．3Hz组及1．OHz组大鼠癫痫发作程度与其无明显差异（P〉0．05），但0．5Hz组及0．8Hz组癫痫发作程度较其明显减轻，癫痫严重度评分显著低于另外3组，差异均有统计学意义（P〈0．05）。结论0．3～1．0Hz低频TMS预处理均可有效延长大鼠癫痫发作潜伏期，并且以0．5Hz及0．8Hz低频TMS的抗癫痫作用较为显著。Objective To study the effects of low-frequency transcranial magnetic stimulation （TMS） on rats with seizures induced by lithium-pilocarpine. Methods Rats were randomly divided into five groups and pretreated with TMS at 75% of the stimulator＇s maximum output, but at 0 Hz （ the sham group）, 0.3 Hz, 0.5 Hz, 0.8 Hz and 1.0 Hz, respectively. After stimulation every day for 2 weeks, a model of acute epilepsy was induced using lithium-piloearpine. Subsequent behavior and the latencies of seizures were observed, and the severity of the seizures was quantitatively evaluated for 90 min. Results The seizure latencies were significantly prolonged in all the other groups Relative to the sham group （P≤0.05）. The prolongation in the 0.5 Hz and 0.8 Hz groups was significantly greater than in the 0.3 Hz and 1.0 Hz groups. The severity of seizures in the 0.3 Hz and 1.0 Hz groups was not significantly different from that in the sham group, but most of the rats in the 0.5 Hz group and the 0.8 Hz group experienced much lighter seizures, with seizure severity scores significantly lower than those in the other 3 groups. Conclusions Pretreatment with TMS at 0.3 to 1.0 Hz led to a longer latency of seizure onset. 0.5 Hz and 0.8 Hz gave the longest latencies and showed conspicuous antiepileptic effects.

关 键 词：经颅磁刺激 癫痫 潜伏期 痫性发作 锂-匹鲁卡品 

分 类 号：R742.1[医药卫生—神经病学与精神病学]