糖尿病大鼠甲状腺组织NISmRNA表达的变化  

Change of NIS mRNA Expression in Thyroid of DM Rats

作　　者：赵伟[1] 朱梦瑜[2] 郑凝[3] 兰毅[1] 马丽云[1] 闫雪[1] 张宏[1] 方佩华[4] 

机构地区：[1]天津医科大学代谢病医院、卫生部和天津市激素与发育重点实验室,300070 [2]天津医科大学基础医学院 [3]天津泰达医院 [4]天津医科大学总医院核医学科

出　　处：《天津医药》2009年第4期289-291,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30670992);天津市自然科学基金资助项目(项目编号:06YFJMJC07800)

摘　　要：目的:探讨糖尿病(DM)大鼠甲状腺组织钠/碘转运体(NIS)mRNA表达的变化及胰岛素、氨基胍对其的干预作用。方法:高脂饲料喂养联合链脲佐菌素制备DM大鼠49只,随机分为DM组17只、胰岛素干预组(DI)16只、氨基胍干预组(DA)16只,设正常对照组(NC)9只,成模12周末处死,取甲状腺组织,以半定量RT-PCR方法测定NISmRNA的表达。结果:DM组大鼠甲状腺组织NISmRNA与GAPDH mRNA表达水平比为0.17±0.09显著低于NC组的0.47±0.16(P<0.05),经胰岛素或氨基胍干预后DI组与DA组大鼠甲状腺组织NIS mRNA与GAPDH mRNA表达水平比分别为0.45±0.20、0.40±0.17显著高于DM组(P<0.05)。结论:高糖毒性可能通过非酶糖基化途径导致DM大鼠甲状腺组织NISmRNA表达下降。Objective： To discuss the expression changes of sodium iodide symporter （NIS） in the thyroid of diabetes mellitus （DM） as well as the intervention effects of insulin and aminoguanidine. Methods： Forty-nine DM rat models were established by high-lipid feedstuff breeding and streptozocin injection into vena caudalis. The DM rat models were divided randomly into DM group （n=lT）, insulin intervention group （n=16） and aminoguanidine intervention group （n=16）. Nine normal rats were taken as controls. Twelve weeks after DM rat model establishment, rats were sacrificed and thyroids were taken to determine NIS mRNA expression with RT-PCR. Results： NIS mRNA/GAPDH mRNA expression ratio in the thyroid of DM rats （0.17± 0.09） was significant lower than that in control group （0.47±0.16）（P 〈 0.05 ）, and NIS mRNA/GAPDH mRNA expression ratio in the thyroid of insulin intervention group （0.45±0.20） and aminoguanidine intervention group （0.40±0.17） were significant higher than that in DM group （P 〈 0.05）. Conclusion： Hyperglycemia toxicity may lead to the decreaseed expression of NIS mRNA in the thyroid of DM rats through non-enzymic glycosylation pathway.

关 键 词：糖尿病 甲状腺 钠 碘 协同转运子 RNA 信使 胰岛素 胍类 大鼠 WISTAR 

分 类 号：R734.2[医药卫生—肿瘤] R581.1[医药卫生—临床医学]