羊膜移植于结膜下的转归  

Outcome of amniotic membrane transplanted under rabbit conjunctiva

作　　者：李娜[1] 赵敏[1] 

机构地区：[1]重庆医科大学附属第一医院眼科,重庆400016

出　　处：《重庆医科大学学报》2009年第4期397-399,共3页Journal of Chongqing Medical University

基　　金：重庆市科委科学基金资助(2003-23)。

摘　　要：目的:研究羊膜移植于结膜下的转归并探讨影响因素。方法:取20只新西兰大白兔,将DTAF(Dichlorotriazinyl amino fluoresc1in,DTAF)标记的羊膜埋植于兔结膜组织下。术后进行临床观察,并于3、5、8、12、16周取材做HE染色观察组织病理学改变,以及激光共聚焦显微镜检查追踪羊膜踪迹。结果:HE染色术后3周羊膜组织结构完整,羊膜前与结膜、筋膜毗邻,羊膜后靠近巩膜;术后5周羊膜仍成条带状,开始溶解;术后8周残留羊膜碎片;术后12周羊膜基本溶解,局部残留疏松淡红色组织;术后16周羊膜完全溶解、消失。炎性反应细胞以浆细胞、单核一巨噬细胞和淋巴细胞为主,术后3周炎症反应达高峰,随后炎性细胞数目逐渐减少,呈慢性炎症反应。激光共聚焦显微镜发现羊膜在结膜下可以存留12周。结论:羊膜移植于结膜下存留时间可长达12周,最终发生溶解、吸收。Objective：To investigate the turnover and influencing factors for amniotic membrane transplantation under conjunctiva. Methods ： Amniotic membrane stained with DTAF was implanted under conjunctiva of 20 New Zealand white rabbits. After transplantation, we observed the clinical change. The tissue samples from grafted area were observed by HE staining and under laser confocal microscope to trace amniotic membrane on 3,5,8,12, 16 W after surgery. Results：The amniotic membrane was intact until 3W after transplantation. The amniotic membrane was at the bottom of the conjunctiva and fascia,near the top of the sclera. At the 5th week after surgery, the amniotic membrane was bandshaped and began dissolved. Remnants of the amniotic membrane could be identified at the 8th week after surgery. After 12 W, the amniotic membrane basically dissolved, but remnants of curmbly erythroic tissue were locally left. The amniotic membrane totally dissolved after 16W. The types of inflammatory cells were mainly plasma ceils, mononucleimacrophages and lymphocytes. At the 3th week after surgery, the inflammatory reaction reached the peak, then the inflammatory cells decreased. The main pathologic reaction was chronic inflammation.Laser confocal microscope discovered that the amniotic membrane persisted for 12 weeks under conjunctiva. Conclusion：The amniotic membrane implanted under conjunctiva persisted for 12 weeks and finally dissolved.

关 键 词：羊膜移植 结膜 转归 

分 类 号：R779.6[医药卫生—眼科]