Prediction of HLA-A 2.1-restricted CTL epitopes from IGFBP7 antigen of lung carcinoma  

作　　者：Zhao Weipeng Long Haixia Zhu Bo Duan Yuzhong Chen Zhengtang 

机构地区：[1]Cancer Research Center, Xinqiao Hospital Third Military Medical University, Chongqing 400037, China [2]School of Bioengineering, Chongqing lnstitute of Technology, Chongqing 400050, China

出　　处：《Journal of Medical Colleges of PLA(China)》2009年第2期63-68,共6页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the Special fund of the National High Technology Research and Development Program of China (863 Program,2007AA02Z129);the National Natural Science Foundation of China (30672076 and 30800506)

摘　　要：Objective： With the development of peptide-based cancer specific immunotherapy, the prediction of CTL epitopes from insulin-like growth factor-binding protein 7 （IGFBP7） is very important for some research about tumor metastasis. Because HLA-A2.1-expressing individuals cover 〉50% in the population of China, we aimed at identifying IGFBPT-encoded peptide presented by HLA-A2.1. Methods： In our study, a HLA-A2.1 restricted CTL epitope was identified by using the following two-step procedure： （a） computer-based epitope prediction from the amino acid sequence of IGFBP7 antigen; （b） Validation with epitope molecular modeling. Results： We obtained four epitopes with high immunogenicity scores by all of the three algorithms, i.e., BIMAS, SYFPEITH1 and IMTECH. Each of the four candidates satisfied the criteria of the HLA-A2.1- restricted CTL epitopes in molecular modeling analysis. Conclusion： The combination of BIMAS, SYFPEITHI and IMTECH method can improve the prediction efficiency and accuracy. Due to this research herein, this four epitopes have potential value for further studied, also have potential application in peptide-mediated immunotherapy. These epitopes may be useful in the design of therapeutic peptide vaccine for lung carcinoma and as immunotherapeutic strategies against lung carcinoma after identified by immunology experiment.Objective:With the development of peptide-based cancer specific immunotherapy,the prediction of CTL epitopes from insulin-like growth factor-binding protein 7(IGFBP7) is very important for some research about tumor metastasis.Because HLA-A2.1-expressing individuals cover >50% in the population of China,we aimed at identifying IGFBP7-encoded peptide presented by HLA-A2.1.Methods:In our study,a HLA-A2.1 restricted CTL epitope was identified by using the following two-step procedure:(a) computer-based epitope prediction from the amino acid sequence of IGFBP7 antigen;(b) Validation with epitope molecular modeling.Results:We obtained four epitopes with high immunogenicity scores by all of the three algorithms,i.e.,BIMAS,SYFPEITHI and IMTECH.Each of the four candidates satisfied the criteria of the HLA-A2.1-restricted CTL epitopes in molecular modeling analysis.Conclusion:The combination of BIMAS,SYFPEITHI and IMTECH method can improve the prediction efficiency and accuracy.Due to this research herein,this four epitopes have potential value for further studied,also have potential application in peptide-mediated immunotherapy.These epitopes may be useful in the design of therapeutic peptide vaccine for lung carcinoma and as immunotherapeutic strategies against lung carcinoma after identified by immunology experiment.

关 键 词：Insulin-like growth factor-binding protein 7 EPITOPE Cytotoxic T lymphocyte 

分 类 号：R377.11[医药卫生—病原生物学] R392.11[医药卫生—基础医学]