新方法简便高效建立小鼠原位膀胱癌模型  

A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer

作　　者：梁中锟[1] 张琳[2] 胡志明[3] 陈忠[1] 黄鑫[3] 石向华[4] 谭万龙[1] 高基民[2,3] 

机构地区：[1]南方医科大学南方医院泌尿外科,广东广州510515 [2]温州医学院浙江省医学遗传学重点实验室,浙江温州325035 [3]南方医科大学生命技术学院生物治疗研究所,广东广州510515 [4]南方医科大学珠江医院肾移植科,广东广州510282

出　　处：《南方医科大学学报》2009年第4期627-630,634,共5页Journal of Southern Medical University

基　　金：国家863高技术研究发展计划(2006AA02Z4C4)

摘　　要：目的用物理化学的方法建立小鼠原位表浅膀胱癌模型,并对影响成瘤率的因素进行探讨,筛选出高效简便建立小鼠原位表浅膀胱癌模型的方法。方法用改造过的静脉留置针经小鼠尿道插入膀胱腔内,实验组用酸碱腐蚀的方法对小鼠膀胱粘膜进行预处理,按观察目的的不同分为4组;对照组不作预处理。PBS冲洗后将MB49灌注入膀胱,构建小鼠原位表浅膀胱癌模型。所有小鼠观察一般情况和肿瘤生长情况。按计划处死小鼠并解剖观察膀胱肿瘤生长情况及有无转移,取标本做病理切片。结果病理显示膀胱粘膜已预处理的小鼠接种肿瘤细胞后可在膀胱部位成瘤(成瘤率100%);丝裂霉素能显著减缓膀胱肿瘤的生长(P=0.000,P<0.05);对照组接种肿瘤细胞后未见成瘤。结论膀胱粘膜处理的时间以酸20s,碱5s较合适;简便的物理化学方法成功建立了可靠性、稳定性和重复性均好的小鼠原位表浅膀胱癌模型,为表浅膀胱癌术后复发的防治研究尤其是抗癌药物的筛选和免疫治疗研究提供了理想的动物实验模型。Objective To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer. Methods C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter （24 G）. The mice were intravesically pretreated with HCI and then with NaOH, and after washing the bladders with phosphate-buffered saline （PBS）, 100 μl （1×10^7） MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C （MMC） administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed. Results Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS. Conclusion We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.

关 键 词：膀胱肿瘤 原位模型 C57BL/6小鼠 MB49 丝裂霉素 

分 类 号：R-332[医药卫生] R737.14