血管紧张素1-7对大鼠肝星状细胞α-平滑肌肌动蛋白表达的影响  

Effect of angiotensin1-7 on α-smooth muscle actin protein expression in rat hepatic stellate cells

作　　者：英嵩崧[1] 李旭[2] 黄茂梁[1] 孟莹[3] 张振书[1] 

机构地区：[1]南方医科大学南方医院消化内科,广东广州510515 [2]南方医科大学南方医院急诊科,广东广州510515 [3]南方医科大学南方医院呼吸内科,广东广州510515

出　　处：《南方医科大学学报》2009年第4期732-733,737,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30871155)

摘　　要：目的探讨血管紧张素Ⅱ(AngⅡ)和血管紧张素1-7(Ang1-7)对大鼠肝星状细胞α-平滑肌肌动蛋白表达的影响。方法采用HSC-T6细胞株,分别给予AngⅡ,Ang1-7,AngⅡ+Ang1-7,Ang1-7+A779 10μmol/L处理,逆转录聚合酶链反应检测Rock通路中Rock2(Rhokinase 2)mRNA的表达。免疫印迹法检测α-平滑肌肌动蛋白的表达水平。结果AngⅡ处理组Rock2mRNA的表达显著增强,Ang1-7可抑制AngⅡ诱导的Rock2mRNA的表达。AngⅡ可诱导α-平滑肌肌动蛋白水平的变化,Ang1-7可抑制AngⅡ诱导的α-平滑肌肌动蛋白表达量。结论Ang1-7可抑制AngⅡ诱导的α-平滑肌肌动蛋白表达。Objective To investigate the effect of angiotensin Ⅱ and angiotensin1-7 on α-smooth muscle actin （α-SMA） -induced C^2＋-independent pathways mediated by Rho kinase2 in hepatic stellate cells （HSCs）. Methods HSC-T6 cells were treated with 10 μmol/L ofAngⅡ, Ang1-7, Ang Ⅱ ＋Ang1-7, and Ang1-7＋A779. RT-PCR was used to detect the expression of Rho kinase2 （Rock2） in Ca2＋-independent pathways, and α-SMA protein expression was detected by Western blotting. Results The mRNA expression of Rock2 increased significantly in the cells alter AnglI treatment （P〈0.01）, but decreased following Ang1-7 treatment. Ang1-7 treatment significantly reduced α-SMA level in AngⅡ-induced cells （P〈0.01）. Conclusion Ana1-7 can inhibit Anvil-induced activation of Rock2 and reduce α-SMA exnression in HSCs.

关 键 词：血管紧张素 ROCK2 信号通路 肝星状细胞 Α-SMA 

分 类 号：R575.2[医药卫生—消化系统]