RNAi抑制卵巢癌SKOV_3细胞MMP-24基因表达及对细胞侵袭的影响  

Inhibitory effects of RNA interference on MMP-24 expression and invasiveness of ovarian cancer SKOV_3 cells

作　　者：骆亚平[1] 钟梅[1] 汪丽萍[1] 孙桂芹[1] 黎静[1] 

机构地区：[1]南方医科大学南方医院妇产科,广东广州510515

出　　处：《南方医科大学学报》2009年第4期781-784,共4页Journal of Southern Medical University

摘　　要：目的探讨应用RNA干扰技术沉默MMP-24基因表达,研究其对人卵巢上皮癌SKOV3细胞侵袭力的影响。方法设计合成2条特异性针对MMP-24基因的siRNA,转染至SKOV3中,采用RT-PCR和Western blotting检测MMP-24 mRNA和蛋白表达情况,细胞侵袭实验观察转染后细胞侵袭能力的变化。结果转染siRNA后,卵巢癌细胞MMP-24 mRNA表达受抑制,蛋白表达降低,细胞侵袭力减弱。结论RNAi沉默MMP-24基因可影响卵巢癌细胞的侵袭,有可能成为治疗卵巢癌的新途径。Objective To investigate the inhibitory effect of RNA interference （RNAi） on MMP-24 expression and invasiveness of ovarian cancer SKOV3 cells. Methods Two pairs of small interfering RNA （siRNA） specific to MMP-24 mRNA were designed and transfected into SKOV3 cells. RT-PCR and Western blotting were used to detect the mRNA and protein expressions of MMP-24, and the cell invasiveness was assessed using an in vitro invasion test. Results After transfection with siRNA, the mRNA and protein expression levels of MMP-24 were obviously reduced in SKOV3 cells, which also showed significantly decreased invasiveness in vitro. Condusion MMP-24 gene silencing by RNAi can suppress the invasiveness of ovarian cancer SKOV3cells in vitro,which may provide a new therapeutic approach of ovarian cancer.

关 键 词：RNA干扰 MMP-24卵巢肿瘤 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]