大中华鳋感染引起草鱼鳃粘液成分的变化  

Changes of Gill Mucus from Ctenopharyngodon idellus Infected with Sinergasilus major

作　　者：刘国勇[1] 

机构地区：[1]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《安徽农学通报》2009年第8期20-20,139,共2页Anhui Agricultural Science Bulletin

基　　金：三峡大学博士启动基金;国家自然科学基金项目(50639070)资助

摘　　要：采用聚丙烯酰胺凝胶电泳和明胶酶谱分析,对感染大中华鳋的草鱼与未感染大中华鳋的健康草鱼鳃粘液进行比较分析。结果表明:健康草鱼和感染大中华鳋的草鱼鳃粘液图谱中有2条带出现差异,感染大中华鳋的草鱼鳃粘液在32kD-34kD处具有蛋白酶活性,而未感染大中华鳋的草鱼鳃粘液在32kD-34kD处没有蛋白酶活性。By using the method of polyacrylamide gel electrophoresis （PAGE） and gelatin zymography, the changes of gill mucus from grass carp （Ctenopharyngodon idellus） infected with parasitic copepods Sinergasilus major was analyzed. Two differential bands were detected in PAGE profiles of gill mucus from noninfected and infected grass carp. In gelatin zymography profiles, one protease band appeared in the gill mucus from infected grass carp with molecular weight of 32-34kD, while no enzyme activity was detected in parallel band in the gill mucus from non-infected grass carp.

关 键 词：草鱼 大中华鳋 粘液 

分 类 号：S851.34[农业科学—预防兽医学]