双液相体系下对黑根霉C_(11)α-羟基化16α,17α-环氧孕酮的研究  

Studies on the Reaction of C_(11)α-Hydroxylation of 16α,17α-Epoxy Progesterone by Rhiz opus Nigricans in the Two-Liquid-Phase System

作　　者：万金营[1] 杜连祥[1] 巩培[1] 武薇[1] 

机构地区：[1]工业微生物教育部重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2009年第2期13-16,共4页Journal of Tianjin University of Science & Technology

基　　金：国家科技基础条件平台资助项目(2005DKA21204-10)

摘　　要：研究了双液相体系下黑根霉对16α,17α-环氧孕酮C11α-羟基化的生物转化能力.考察了8种有机溶剂对16α,17α-环氧孕酮和C11α-羟基-16α,17α-环氧孕酮的溶解度的差异,比较了它们对黑根霉细胞毒性的大小.结果表明,乙酸丁酯毒性最小,乙酸丁酯与水双液相体系对转化比较有利,达到了44.2%.同时考察了氧促进剂H2O2和外加电子受体维生素K3对提高该体系转化率的影响,分别提高了8%和6%.The biotransformation of C11α-hydroxylation 16α, 17α-epoxy progesterone by Rhizopus nigricans in the two- liquid-phase fermentation system was studied. Eight kinds of organic solvents were chosen ,their toxicity were compared and their solubility to 16α, 17 α-epoxy progesterone and C11 α-hydroxyl- 16α, 17α-epoxy progesterone were also researched. The toxicity of butyl acetate is in the lowest level and the conversion ratio （44.2%） is higher than other two-liquid-phase systems. The effect of H2O2 and vitamin K3 on increasing conversion ratio in this system were also studied,reaching to 8% and 6%, respectively.

关 键 词：黑根霉 C11α-羟基化 16α 17α-环氧孕酮 双液相发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]