马鞭草诱导人绒毛膜癌JAR细胞凋亡作用观察  

OBSERVATION ON THE INHIBITION EFFECT OF VERBENA OFFICINALIS ON HUMAN CHORIOCARCINOMA JAR CELLS

作　　者：张立平[1] 徐昌芬[2] 

机构地区：[1]安徽马鞍山市中心医院,马鞍山243000 [2]南京医科大学

出　　处：《现代预防医学》2009年第8期1523-1527,共5页Modern Preventive Medicine

基　　金：江苏省自然科学基金资助项目(BK2003104);马鞍山市科技局资助项目(2004-24)

摘　　要：[目的]探讨马鞭草C部位对人绒癌JAR细胞抑制作用机理。[方法]通过相差显微镜观察活细胞贴壁程度、细胞形态改变;荧光显微镜观察细胞形态学变化;透射电镜观察细胞超微结构变化;琼脂糖凝胶电泳观察用药后细胞DNA断裂情况;流式细胞仪检测细胞凋亡情况。[结果]马鞭草C部位可引起细胞数目减少并萎缩;荧光显微镜下观察到典型凋亡细胞的形态学特征;电镜下见到明显的凋亡小体;琼脂糖凝胶电泳出现"阶梯状条带";流式细胞仪检测发现用药后出现明显的凋亡峰。[结论]马鞭草C部位对人绒癌JAR细胞抑制作用机理是诱导其凋亡。[Objective] To investigate the inhibition mechanism of Verbena officinalis C on Human Choriocarcinoma JAR Cells. [Methods] Morphological changes and adherence degrees of JAR cells were observed with contrast phase microscope and fluorescence microscope; The ultrastructure changes of cells were also observed by transmission electron microscope; The DNA fracture status of cells were observed by agarose gel electrophoresis; the cell cycle and apoptosis was analyzed by FCM. [Results] The incubated tumor cells were decreased in number and became atrophic when they were treated with Verbena officinalis C; The typical apoptotic morphology was observed by fluorescence microscope; There was obvious apoptosis body under the electron microscope; The cascade strip was found after detecting by DNA gel electrophoresis, and obvious apoptotic peak was detected by flow cytometer after treating with Verbena officinalis C. [Conclusion] The inhibition mechanism of Verbena officinalis C on Human Choriocarcinoma JAR Cells was to induce apoptosis.

关 键 词：马鞭草 绒毛膜癌 JAR细胞 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]