十二指肠胃食管反流动物模型的改进和完善  

Modification of animal model of duodenum gastroesophageal reflux

作　　者：曹丽霞[1] 袁红霞[1] 杨幼新[1] 代二庆[2] 赵强[3] 

机构地区：[1]天津中医药大学,研究生天津300193 [2]武警医学院附属医院,天津300162 [3]天津中医药大学第一附属医院,天津300193

出　　处：《天津中医药》2009年第2期134-136,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30740063);天津市卫生局中医中西医结合课题课题(07014)

摘　　要：[目的]改进"食管十二指肠端侧吻合术",完善十二指肠胃食管反流大鼠模型的构建。[方法]模型组(A组)采用"四壁四针"间断吻合术式,对照组(B组)采用改良后的"食管十二指肠端侧吻合术"。[结果]模型组和对照组食管下段黏膜pH值差异无显著性意义(P>0.05),模型组大鼠成活率(85%)较对照组(65%)有显著性差异(P<0.05),模型组大鼠食管下端黏膜肉眼表现较对照组有显著性差异(P<0.05),模型组大鼠食管下段病理改变与对照组差异无显著性(P>0.05)。[结论]改进术式的模型在增加存活率的基础上成功的诱发了反流性食管炎及Barrett’s食管。[Objective] To improve the ＂Esophagus duodenum end-to-side anastomosis technology＂ and perfect the animal model of Duodenal gastroesophageal reflux. [Methods] In Model group （A） the ＂Four walls four pins＂ of the author was adopted while in the control group （B） the end-to side esophagus duodenum anastomosis modified was adopted. [Results] There was no difference in pH values of mucous membrane at the lower esophageal segment between the two groups （P〉0.05）. The survival rate of rats in model （85%） and the control group （65%） was significantly different （P〈0.05）. There was significant difference between the two groups on the performance of esophageal mueosa（P〈0.05）, but no difference on pathological changes（P〉0.05）. [Conclusion] The improved model can successfully induce the reflux esophagitis and Barrett＇ s esophagus on the basis of increasing in survival rate.

关 键 词：食管炎 十二指肠胃食管反流 模型 大鼠 

分 类 号：R574.51[医药卫生—消化系统]