姜黄素对人宫颈癌SiHa细胞株增殖、凋亡和COX-2表达的影响  

Effect of curcumin on preliferation,apoptosis and COX-2 expression of human cervical cancer SiHa cell

作　　者：瞿秋红[1] 付翠敏[1] 韦立蓓[2] 李华[1] 尹伶[1] 

机构地区：[1]武汉科技大学临床学院妇产科,湖北武汉430064 [2]湖北大悟县人民医院妇产科

出　　处：《中国妇幼保健》2009年第12期1683-1685,共3页Maternal and Child Health Care of China

基　　金：武汉科技大学校内基金(项目编号:2006XY41)

摘　　要：目的:体外研究中药姜黄素(Cur)对子宫颈癌SiHa细胞的增殖抑制和诱导凋亡的作用及与环氧合酶(COX-2)表达的关系。方法:以不同浓度的姜黄素(10～30μmol/L),分12～72 h四个时间点处理SiHa细胞,光镜观察细胞形态变化;用四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率;用DNA梯状电泳(DNA ladder)检测凋亡的发生;Western blot检测COX-2蛋白的表达;用放射免疫法检测细胞PGE2释放水平。结果:姜黄素可抑制SiHa细胞增殖,作用呈明显的时效和量效关系,差异有统计学意义(P<0.01);姜黄素可诱导SiHa细胞凋亡,DNA ladder呈梯状条带;姜黄素可明显抑制COX-2的表达,差异有统计学意义(P<0.01),并呈浓度依赖性;姜黄素明显抑制SiHa细胞PGE2释放水平,差异有统计学意义(P<0.01)。结论:姜黄素体外对SiHa细胞具有增殖抑制作用和促进凋亡作用,其机制可能与抑制COX-2蛋白表达、降低PGE2释放水平有关。Objective：To explore the SiHa cells growth inhibition and apoptosis induced by curcumin and the relationship with COX-2 expression in vitro.Methods：SiHa cells were treated with various concentrations（10～30 μmol/L） of curcumin for 12～72 hours.Cell changing was observed by microscope,the inhibition rate of cell proliferation was measured by MTT method;apoptosis was detected by DNA ladder.The level of PGE2 was measured by radioimmunoassay.The expression of COX-2 protein was also examined by Western blot.Results：The cell growth of SiHa reduced after treated with curcumin,showing dose-and time-dependent manner in proliferative inhibition rate（P〈0.01）.The curcumin induced apoptosis,DNA fragmentation gel analysis showed DNA ladder.Curcumin reduced the expression of COX-2（P〈0.01）,showing concentration dependent manner;curcumin reduced the release of PGE2 in SiHa cells（P〈0.01）.Conclusion：Curcumin can inhibit the proliferation and promote the apoptosis in human SiHa cells in vitro,which is related to the inhibition of COX-2 expression and the decrease of PGE2 release.

关 键 词：姜黄素 环氧合酶-2 SIHA细胞 凋亡 

分 类 号：R73-74[医药卫生—肿瘤] R737.33[医药卫生—临床医学]