秸秆降解菌株的筛选及其发酵生产乙醇的研究  

SCREENING STRAW-DEGRADING MICROBIAL STRAINS AND THE STUDY OF FUEL-ETHANOL FERMENTATION

作　　者：赵磊[1,2] 马沛[1,2] 范代娣[1,2] 骆艳娥[1,2] 马晓轩[1,2] 

机构地区：[1]西北大学化工学院,西安710069 [2]陕西省生物材料与发酵工程技术研究中心,西安710069

出　　处：《太阳能学报》2009年第4期521-525,共5页Acta Energiae Solaris Sinica

摘　　要：用滤纸赫奇逊固体培养基和纤维素固体培养基初筛,再以滤纸无机盐培养基和以羧甲基纤维素为唯一碳源的刚果红培养基进行复筛的方法,确定出10株降解效果较好的菌,通过对其纤维素酶相关性质及酶活力进行测定,综合比较降解能力,最终获得6株降解活性较强的菌,其酸性纤维素酶活较强,最优产酶时间为6d,最后采取了非等温同时糖化发酵法(Nonisothermal Simultaneous Saccharification and Fermentation,NSSF)和同步糖化发酵法(SimultaneousSaccharification and Fermentation,SSF)进行乙醇发酵,结果表明NSSF的乙醇产率大于SSF。Fuel-ethanol production by lignocellulose is a trend of energy development. Lignocellulose was degraded into sugars, and then ethanol was produced by fermentation. The aim of this paper was to screen strains with the strong decomposition ability of cellulose, and study the ethonal fermentation. In this trial, 10 strains were obtained by screening using filter-paper Hutchison solid medium and CMC solid culture medium, and then rescreening by filter-paper inorganic salt culture medium and Congo red culture medium with the CMC-Na as the only carbon source. After testing the characters of cellulase and comparing enzyme activities, 6 strains with high degrading efficiency were obtained finally, their activity of acidic cellulase was higher, and the optimal output time of enzyme was the sixth day. At ethanol fermentation stage, the production of ethanol with nonisothermal simultaneous saccharication and fermentation （NSSF） and simultaneous saccharification and fermentation（SSF） was studied, and the results showed the ethanol yield of NSSF was higher than that of SSF.

关 键 词：筛选 降解 木质纤维素 燃料乙醇 发酵 

分 类 号：TQ352[化学工程]