口腔鳞癌发生过程中Survivin促血管生成作用的研究  

Expression of Survivin and its correlation with tumor angiogenesis in oral squamous cell carcinoma

作　　者：李曙霞[1] 丁艳平[1] 张辛燕[1] 孙正[1] 

机构地区：[1]首都医科大学口腔医学院,北京100050

出　　处：《北京口腔医学》2009年第2期61-64,共4页Beijing Journal of Stomatology

基　　金：国家自然基金资助项目(NO.30500559);北京市科技新星项目(NO.2005B51)

摘　　要：目的探讨口腔鳞癌发生过程中Survivin促进血管生成的作用。方法选取北京口腔医院病理科存档石蜡标本45例,其中口腔白斑伴上皮轻-中度异常增生16例,口腔白斑伴上皮重度异常增生12例,口腔高-中分化鳞状细胞癌17例,另取正常口腔粘膜组织10例作对照。采用免疫组化SP法对各组织病损中Survivin和VonWillebrandFactor的表达情况进行检测。结果免疫组化结果显示,从正常粘膜→白斑伴上皮轻-中度异常增生→白斑伴上皮重度异常增生→口腔鳞癌,Survivin的表达量呈增加趋势;微血管密度则由正常对照组的28.49±11.87条/mm2升高到口腔鳞癌组的91.98±40.20条/mm2,各组之间有显著性差异。结论Survivin的过度表达是口腔鳞癌发生过程中的早期事件。随着Survivin表达量的增加,微血管密度明显升高。Survivin的过表达可能抑制了血管内皮细胞的凋亡,从而促进血管的生成。Objective To explore the roles of survivin in oral cancer angiogenesis. Methods Archival paraffin sections of 10 normal oral mucosa, 16 leukoplakia with mild to moderate dysplasia, 12 leukoplakia with severe dysplasia and 17 primary squamous cell carcinoma were obtained for immunohistochemical staining of survivin and Von Willebrand Factor. Survivin-positive cells in epithelium and the stroma were counted and analyzed statistically. Results Strong survivin expression was observed in carcinoma and not observed in normal oral mucosa. From oral epithelial precursor lesions to squamous cell carcinoma, the expression of survivin was increased with increasing grade. Von WiIlebrand Factor was also highly expressed in carcinoma- Conclusion Over-expression of survivin is an early event in the development of oral carcinoma and may be involved in angiogenesis of oral carcinoma.

关 键 词：SURVIVIN 口腔鳞癌 微血管密度 

分 类 号：R739.8[医药卫生—肿瘤]